干旱区生态环境知识资源中心(Arid): 超高效液相色谱法同时测定植物油中15种多环芳烃

Arid

	超高效液相色谱法同时测定植物油中15种多环芳烃
其他题名	Simultaneous determination of 15 polycyclic aromatic hydrocarbons additives in vegetable oil by ultra performance liquid chromatography
	董桂贤; 王朝霞; 周晓歌; 张晓瑜; 王颖; 张桂芳
来源期刊	卫生研究
ISSN	1000-8020
出版年	2014
卷号	43 期号:4 页码:620-623
中文摘要	目的建立采用超高效液相色谱(UPLC)-荧光检测器(FLR)同时快速检测植物油中15种多环芳烃的方法。方法采用乙腈∶ 丙酮(1∶ 1)混合溶剂提取,先后使用Waters 的Oasis HLB 和Sep-Pak Florsil 小柱净化,Waters PAH C_(18)色谱柱(4. 6mm * 50 mm,3 mum)分离,甲醇、乙腈和水进行梯度洗脱,柱温35℃,流速0. 80 ml /min,进样量10 mul;荧光检测器采用程序定时控制荧光检测波长变化,外标法定量。结果15种多环芳烃9 min 内完全分离,在2. 0~200. 0 mug /L 范围内,峰面积和质量浓度的线性关系良好(r≥0. 9990),以高、中、低浓度(10、50和100 mug /kg)作为不同的添加水平,平均加标回收率为75. 8%~96. 4%,RSD 为3. 42%~8. 03%(n = 5),检出限为0. 025~0. 8 mug /kg,定量限为0. 08,3. 0 mug /kg。结论该方法操作方便、分离效果好、线性范围宽,能满足植物油中15种多环芳烃的检测要求。
英文摘要	Objective To establish a rapid method for simultaneous determination of 15polycyclic aromatic hydrocarbons (PAHs) in vegetable oil by ultra performance liquid chromatography with fluorescence detection. Methods The vegetable oils were extracted with acetonitrile and acetone(1∶ 1),purified with Oasis HLB and Sep-Pak Florsil column, separated on Waters PAH C_(18) (4. 6 mm * 250 mm,3 mum) special column for the analysis of polycyclic aromatic hydrocarbons with a mobile phase of acetonitrile,methanol and water for gradient elution,the column temperature was 35 ℃,and the injection was 10 mul. The concentration of PAHs in samples were determined with fluorescence detector,and quantitative analysis was carried out by external standard. Results The 15 PAHs were completely separated within 9 min. Within 2 to 200 mug /L,the peak area and content was in a good linear relationship (r≥0. 9990). The average recoveries of three spiked levels (10,50 and 100 mug /kg) were 75. 8% and 96. 4%,with RSDs of 3. 42%-8. 03% (n = 5). The limits of detection were 0. 025-0. 8 mug /kg and the limits of quantification were 0. 08-3. 0 mug /kg. Conclusion This method is simple and quick with high sensitivity, and it is suitable for the determination of 15 PAHs in vegetable oil.
中文关键词	超高效液相色谱法 ; 植物油 ; 多环芳烃
英文关键词	ultra performance liquid chromatography vegetable oil polycyclic
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FOOD SCIENCE TECHNOLOGY
WOS研究方向	Food Science & Technology
CSCD记录号	CSCD:5212087
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231859
作者单位	山东省烟台市疾病预防控制中心, 烟台, 山东 264003, 中国
推荐引用方式 GB/T 7714	董桂贤,王朝霞,周晓歌,等. 超高效液相色谱法同时测定植物油中15种多环芳烃[J],2014,43(4):620-623.
APA	董桂贤,王朝霞,周晓歌,张晓瑜,王颖,&张桂芳.(2014).超高效液相色谱法同时测定植物油中15种多环芳烃.卫生研究,43(4),620-623.
MLA	董桂贤,et al."超高效液相色谱法同时测定植物油中15种多环芳烃".卫生研究 43.4(2014):620-623.