干旱区生态环境知识资源中心(Arid): UPLC-MS-MS检测饮料中的4-甲基咪唑及其同分异构体2-甲基咪唑

Arid

	UPLC-MS-MS检测饮料中的4-甲基咪唑及其同分异构体2-甲基咪唑
其他题名	Determination of 4-(5-)-Methylimidazole and Its Isomer 2-Methylimidazole in Beverage by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry
	张璐; 孔祥虹; 何强; 李建华; 邹阳; 李莹
来源期刊	食品科学
ISSN	1002-6630
出版年	2014
卷号	35 期号:8 页码:244-248
中文摘要	建立超高效液相色谱-串联质谱(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS-MS)法检测饮料中的4-甲基咪唑(4-(5-)-methylimidazole, 4-Mel)及2-甲基咪唑(2-methylimidazole, 2-Mel)的方法。以水溶解样品,OASIS?MCX固相萃取柱富集净化后,进入UPLC-MS-MS检测,内标法定量。在优化的条件下,以ACQUITY UPLC?HILIC色谱柱(2.1 mm X 100 mm, 1.7 mum)为分析柱,乙腈和5 mmol/L甲酸铵溶液为流动相,在电喷雾正离子多反应监测模式进行检测。结果表明,4-Mel和2-Mel在9~500 ng/mL范围内线性关系良好。方法的定量限为3.0mug/kg。在4个加标水平下,3种不同基质饮料中4-Mel和2-Mel的平均回收率分别为97.3% 和93.7%,相对标准偏差分别为3.8%和3.4%,该方法适用于饮料中4-Mel和2-Mel的检测。利用该方法对市售饮料中4-Mel和2-Mel的含量进行检测,在软饮料、焦糖色饮料及咖啡饮料中检出含有4-Mel或2-Mel;而功能性饮料中未检出4-Mel和2-Mel。
英文摘要	An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method was developed for the determination of 4-(5-)-methylimidazole (4-Mel) and its isomer, 2-methylimidazole (2-Mel), in beverage. Samples were diluted with water. An OASIS? MCX solid phase extraction column was used to purify and concentrate the target analytes. Under the optimal conditions, the analytes were separated by a Waters ACQUITY UPLC? HILIC column (2.1 x 100 mm, 1.7 mum) using a mobile phase consisting of acetonitrile and 5 mmol/L ammonium acetate aqueous solution. The analytes were detected in multiple-reaction monitoring (MRM) mode via positive electrospray ionization (ESI+). The results showed that the calibration curves of 4-Mel and 2-Mel were of good linearity in the range of 9-500 ng/mL. The limit of quantification of the method was 3.0 mug/kg. The average recovery was 97.3% and 93.7% at four spiked levels (3.0, 25,50 and 75 mug/kg) for 4-Mel and 2-Mel, respectively, and the relative standard deviation (RSD) was 3.8% and 3.4%, respectively. These figures of merit indicated that the developed method can be used for the determination of 4-Mel and 2-Mel in beverage samples. This method was applied to different beverages. 4-Mel or 2-Mel was found in soft drink, caramel colored beverage and coffee. Contrarily, neither of them was found in energy drinks.
中文关键词	超高效液相色谱-串联质谱 ; 4-甲基咪唑 ; 2-甲基咪唑 ; 固相萃取 ; 饮料
英文关键词	ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS-MS) 4-(5-)-methylimidazole 2-methylimidazole solid phase extraction (SPE) beverage
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FOOD SCIENCE TECHNOLOGY
WOS研究方向	Food Science & Technology
CSCD记录号	CSCD:5143958
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231688
作者单位	陕西出入境检验检疫局, 西安, 陕西 710068, 中国
推荐引用方式 GB/T 7714	张璐,孔祥虹,何强,等. UPLC-MS-MS检测饮料中的4-甲基咪唑及其同分异构体2-甲基咪唑[J],2014,35(8):244-248.
APA	张璐,孔祥虹,何强,李建华,邹阳,&李莹.(2014).UPLC-MS-MS检测饮料中的4-甲基咪唑及其同分异构体2-甲基咪唑.食品科学,35(8),244-248.
MLA	张璐,et al."UPLC-MS-MS检测饮料中的4-甲基咪唑及其同分异构体2-甲基咪唑".食品科学 35.8(2014):244-248.