干旱区生态环境知识资源中心(Arid): 若尔盖高原泥炭沼泽土嗜冷细菌系统发育分析

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	若尔盖高原泥炭沼泽土嗜冷细菌系统发育分析
其他题名	Phylogeny of Psychrophilic Bacteria from Peat Swamp Soil in Zoige Plateau
	辜运富; 郑有坤; 王宪斌; 张小平
来源期刊	湿地科学
ISSN	1672-5948
出版年	2014
卷号	12 期号:5 页码:631-637
中文摘要	结合低温纯培养手段、核糖体DNA扩增片段限制性内切酶分析技术和克隆测序,研究了若尔盖高原泥炭沼泽土中可培养菌的多样性、系统发育和产低温酶特性。从3块采样地的土样中分离到47株表型各不相同的菌株。16S rRNA测序和系统发育分析结果表明,若尔盖高原泥炭沼泽土的可培养低温菌高度相似于分离自极地、高山冻土和冰川等低温环境的嗜冷菌。47株菌分属于4个不同的群,包括高GC含量群、低GC含量群、Beta和Gama变形菌纲等。革兰氏阳性菌以节杆菌属(Arthrobacter)为主,而革兰氏阴性菌以假单胞菌属(Pseudomonas)为主。其他的细菌分属于微小杆菌属(Exiguobacterium)、芽孢八叠球菌属(Sporosarcina)、贪噬菌属(Variovorax)、紫色杆菌属(Janthinobacterium)和寡养单胞菌属(Stenotrophomonas)等不同的类群。产低温酶分析结果表明,在分离到的47株菌中,大多数菌株都能分泌纤维素降解酶,50%以上的菌株具有蛋白酶活性,而以节杆菌的胞外酶产生能力最强。若尔盖高原泥炭沼泽土中存在丰富的低温菌类群,在酶学生物技术上具有潜在应用价值。
英文摘要	The samples at depth of 10-20 cm of peat swamp soil, covered by Carex meyeriana, were collected from the pastures in Axirong village, Maixi village and Military subarea in Zoige Plateau in October 2008, and 9 sampling sites were arranged in each sampling pasture. To isolate cold-adapted bacterial strains from the peat swamp soil, bacteria were enriched using aerobic heterotrophic conditions at 4 ℃. Then amplified ribosomal DNA restriction analysis (ARDRA) was applied to analysis phylogeny of the isolates, which could be further used to monitor the potential biotechnical applications of the specific enzymes produced by these bacteria. Totally, 47 strains with different phenotypic characteristics were isolated from the three areas. The majority of the isolates were phylogenetically related to bacteria isolated from various cold environments such as the two Polar Regions, permafrost and glaciers. The isolates represented 4 major groups: the high-GC and the low-GC Gram-positives, and beta- and gamma-Proteobacteria. Among them, 4 representative isolates related (> 97.0%) to the genus Arthrobacter. REGD2 that represented the biggest ARDRA group was most closely related to Arthrobacter sp. GW10-3 isolated from Antarctica, with 99.46% sequence similarity; three isolates, REGD7, REGD15, and REGD16, were highly similar to Arthrobacter sp. strains isolated from Arctic, Antarctic and Himalaya; two representative isolates were grouped in the low-GC Gram-positives: REGD5 was most closely related to Exiguobacterium sibiricum strain 255-15 isolated from Siberian permafrost, with 99.6% sequence similarity; the most similar cultured strain to the other low-GC isolate, REGD8, was Paenisporosarcina macmur? doensis strain HWG-A8 isolated from the Tibetan Plateau permafrost soil, with 99.8% sequence similarity. The Gram-negative isolates are all belong to Proteobacteria, two representative isolates were affiliated with beta-Proteobacteria while the other seven representative isolates related to gamma-Proteobacteria. REGD10 was most similar to Variovorax paradoxus strain IHB B 1313 isolated from the cold deserts of the Indian trans-Himalayas, with 99.4 % sequence similarity, while REGD11 was most closely related to Janthinobacterium sp. HC3-10 from Arctic Cyanobacterial mats, with 99.8% sequence similarity. Five isolates (REGD1, REGD6, REGD9, REGD12, and REGD14) were affiliated with different Pseudomonas strains. The other two isolates (REGD3, REGD4) were affiliated with the genus Stenotrophomonas. However, the most similar cultured strain to both these isolates was Acetobacter pasteurianus strain CCM 3606 with 99.74% and 99.8% sequence similarity, respectively. Most of the isolates (87%) from the plateau wetland soil deposits were psychrotrophic and could not grow at temperatures above 30 ℃, and their optimum growth temperature were between 10-15 ℃. Most of the isolates could produce cellulase and almost half of them showed protease activity. In the extracellular enzyme assays, the Arthrobacter isolates were the most versatile ones. The wide range of enzymatic activities of the isolates suggested that psychrophilic bacteria in Zoige Plateau are a promising source of enzymes for biotechnical applications.
中文关键词	泥炭沼泽土 ; 可培养嗜冷菌 ; 16S rRNA基因 ; 多样性 ; 若尔盖高原
英文关键词	peat swamp soil culturable psychrophilic bacteria 16S rRNA gene diversity Zoige Plateau
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:5287039
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231676
作者单位	四川农业大学资源环境学院微生物系, 成都, 四川 611130, 中国
推荐引用方式 GB/T 7714	辜运富,郑有坤,王宪斌,等. 若尔盖高原泥炭沼泽土嗜冷细菌系统发育分析[J],2014,12(5):631-637.
APA	辜运富,郑有坤,王宪斌,&张小平.(2014).若尔盖高原泥炭沼泽土嗜冷细菌系统发育分析.湿地科学,12(5),631-637.
MLA	辜运富,et al."若尔盖高原泥炭沼泽土嗜冷细菌系统发育分析".湿地科学 12.5(2014):631-637.