干旱区生态环境知识资源中心(Arid): 盐胁迫下沙冬青细胞端粒酶活性的变化与DNA稳定性的关系

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	盐胁迫下沙冬青细胞端粒酶活性的变化与DNA稳定性的关系
其他题名	Effects of Salt Stress on Telomerase Activity in Relation to DNA Stability of Ammopiptanthus mongolicus Cells
	张徐俞 1; 王瑾瑜 2; 郑广顺 3; 张俊琦 1; 卢存福 1
来源期刊	生物技术通报
ISSN	1002-5464
出版年	2014
期号	10 页码:134-138
中文摘要	沙冬青(Ammopiptanthus mongolicus)是亚洲中部荒漠地区的常绿阔叶灌木,是古老的第三纪孑遗植物,具有极强的抗逆能力。旨在探究盐胁迫下沙冬青细胞端粒酶活性变化与染色体DNA稳定性的关系。结果表明,在100 mmol/L NaCl的低浓度盐胁迫处理下,随着处理时间的增加,端粒酶活性没有增加,未出现明显的DNA降解现象;当在500 mmol/L高浓度盐胁迫时,处理的初期端粒酶活性迅速的增加,但随着处理时间的延长,端粒酶活性下降,此时DNA未明显降解;当移除NaCl胁迫,端粒酶活性增加1.4倍,DNA保持稳定。因此推测,在盐胁迫下,沙冬青细胞端粒酶活性的维持对避免细胞遭受不可逆损伤,保持染色体DNA稳定性具有一定的作用。
英文摘要	Ammopiptanthus mongolicus, the evergreen broadleaf shrub indigenous to the northwest desert of China, is a residual plant of the ancient subtropical area in the Tertiary Period and has been identified as the national tertiary protection species. This research was conducted to explore the relationship between DNA damage and telomerase activity of Ammopiptanthus mongolicus cells under salt stress. The results showed that telomerase activity was increased during the first 3 d of low salt(100 mmol/L NaCl)treatment. However, when culture cells were treated with 500 mmol/L NaCl, ?telomerase activity increased rapidly at the initial stage, while declined?after 2 day salt stress. Telomerase activity increased 1.4-fold in the recovery phase when 500 mmol/L NaCl was removed from the growth medium. DNA damage was not obvious during the NaCl treatment time or in the phase when NaCl was removed from the growth medium. It is proposed that plants might have developed a highly efficient DNA repair system to cope with transient salt stress, and telomerase may play an important role in it.
中文关键词	沙冬青 ; 盐胁迫 ; 端粒酶 ; DNA损伤
英文关键词	Ammopiptanthus mongolicus Salt stress Telomerase DNA damage
语种	中文
国家	中国
收录类别	CSCD
WOS类目	BIOLOGY
WOS研究方向	Life Sciences & Biomedicine - Other Topics
CSCD记录号	CSCD:5274583
来源机构	北京林业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231672
作者单位	1.北京林业大学生物科学与技术学院, 分析测试中心林木育种国家工程实验室, 北京 100083, 中国; 2.清华大学分析测试中心, 北京 100084, 中国; 3.中国科学院植物研究所, 北京 100093, 中国
推荐引用方式 GB/T 7714	张徐俞,王瑾瑜,郑广顺,等. 盐胁迫下沙冬青细胞端粒酶活性的变化与DNA稳定性的关系[J]. 北京林业大学,2014(10):134-138.
APA	张徐俞,王瑾瑜,郑广顺,张俊琦,&卢存福.(2014).盐胁迫下沙冬青细胞端粒酶活性的变化与DNA稳定性的关系.生物技术通报(10),134-138.
MLA	张徐俞,et al."盐胁迫下沙冬青细胞端粒酶活性的变化与DNA稳定性的关系".生物技术通报 .10(2014):134-138.