干旱区生态环境知识资源中心(Arid): 石漠化地区豆科植物根瘤菌降解碳酸钙、镁能力研究

Arid

	石漠化地区豆科植物根瘤菌降解碳酸钙、镁能力研究
其他题名	Leguminous Plants Rhizobia Degradation of Calcium Carbonate and Magnesium Carbonate
	王明月 1; 刘绍雄 2; 熊智 1; 丁雅迪 1; 王艳玲 1; 李克艳 1; 阳廷丹 1; 陶茜 1; 王金华 1
来源期刊	生态环境学报
ISSN	1674-5906
出版年	2014
卷号	23 期号:10 页码:1581-1585
中文摘要	微生物对岩溶地区岩石风化、成土具有明显的促进作用,为了筛选出能够促进碳酸盐岩风化、加快成土速率的优势根瘤菌菌株,将其与豆科植物建立共生体系,进行石漠化地区修复与治理,实验以分离自云南石漠化地区的9株豆科植物根瘤菌为研究对象,将9株根瘤菌接种于加有难溶性碳酸钙或碳酸镁的液体培养液中,28.5℃、160 r·min-1摇床培养15d,于0、1、3、6、10、15 d取样2 mL,每个样3个平行;将样品加入到10 mL体积比5 : 1的硝酸高氯酸消化液中浸泡过夜,放在电热板上缓慢加热至溶液澄清透明,分别用含La~(3+)2%的盐酸溶液定容至10mL,空白用同样方法处理;最后采用原子火焰吸收方法测定样品中Ca~(2+)和Mg~(2+)含量,筛选出对碳酸盐岩主要成分碳酸钙和碳酸镁具有强降解作用的根瘤菌菌株。结果表明:热带根瘤菌Rhizobium tropici (SWFU09)和根瘤菌Rhizobium sp. (SWFU02)对碳酸钙降解作用最强;对碳酸镁降解作用最好的菌株为根瘤菌 Rhizobitm sp. (SWFU03)、热带根瘤菌 Rhizobium tropici (SWFU05)和慢生根瘤菌 Bradyrhizobium sp. (SWFU01)。溶液pH值对碳酸镁降解作用具重要的影响,对碳酸钙降解作用影响不大,说明根瘤菌代谢产生的酸性物质(有机酸、氨基酸)对碳酸钙和碳酸镁降解作用有一定影响,但不是唯一影响因素;也可能是微生物代谢产生的有机配体(基)吸附碳酸钙和碳酸镁表面,与水体中的成分形成复合物,改变溶解的平衡,置换出Ca~(2+)和Mg~(2+)。
英文摘要	Microorganism played an obvious role in facilitating the reaction rate of rock weathering and soil formation, in order to attain dominant rhizobium strains promoted the rock weathering and speed up the rate of soil rhizobium, the dominant rhizobium strains established the symbiotic system with leguminous plants to repair and manage the rocky desertification areas, the thesis studied on the 9 strains from leguminous plants rhizobia by the test of doing the strains in liquid medium with difficult soluble calcium carbonate or magnesium carbonate, then, handled with 28.5 ℃, 160 r min~(-1) and 15 d table culture, sampled 2 ml of three at 0, 1,3,6,10, 15 d. Samples added into the 10 mL digestive juices for a night, nitric acid and perchloric acid volume ratio was 5 to 1 of the digestive juices, heating Slowly, get clear solutions, the capacity to 10 mL by adding 2% hydrochloric acid solution which contain La~(3+),the same do with blank, used the flame atomic absorption method to determin contents of Ca~(2+) and Mg~(2+) in the samples parallel. We eagered to obtain the certain strains had strong degradation of calcium carbonate or magnesium carbonate. The results showed that the best calcium carbonate degradation strains were Rhizobium tropici (SWFU09) and Rhizobium sp. (SWFU02), the best strains of magnesium carbonate degradation were Rhizobium sp. (SWFU03), Rhizobium tropici (SWFU05) and Bradyrhizobium sp. (SWFU01). The solution pH had an important influence on degradation of magnesium carbonate, but not obvious on calcium carbonate degradation. We could have a conclusion: acid producted by microbial metabolism had a certain influence on degradations of calcium carbonate and magnesium carbonate, but it was not the only factor. Organic ligand (base) also producted by microbial metabolism might adsorpt on calcium carbonate or magnesium carbonate surface, it could form compounds with ingredients in the water, change the balance of dissolution, Ca~(2+) and Mg~(2+)were replaced.
中文关键词	根瘤菌 ; 降解作用 ; 碳酸钙 ; 碳酸镁
英文关键词	rhizobia degradation calcium carbonate magnesium carbonate
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MICROBIOLOGY
WOS研究方向	Microbiology
CSCD记录号	CSCD:5296672
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231551
作者单位	1.西南林业大学, 西南山地森林资源保护与利用云南省省部共建教育部重点实验室, 昆明, 云南 650224, 中国; 2.中华全国供销合作总社昆明食用菌研究所, 昆明, 云南 650223, 中国
推荐引用方式 GB/T 7714	王明月,刘绍雄,熊智,等. 石漠化地区豆科植物根瘤菌降解碳酸钙、镁能力研究[J],2014,23(10):1581-1585.
APA	王明月.,刘绍雄.,熊智.,丁雅迪.,王艳玲.,...&王金华.(2014).石漠化地区豆科植物根瘤菌降解碳酸钙、镁能力研究.生态环境学报,23(10),1581-1585.
MLA	王明月,et al."石漠化地区豆科植物根瘤菌降解碳酸钙、镁能力研究".生态环境学报 23.10(2014):1581-1585.