干旱区生态环境知识资源中心(Arid): 超高效液相色谱-串联质谱法同时测定田螺中3种微囊藻毒素

Arid

	超高效液相色谱-串联质谱法同时测定田螺中3种微囊藻毒素
其他题名	Simultaneous UHPLC-MS/MS Determination of 3 Microcystic Algae Toxins in Field Snail
	沈坚; 张爱芝; 曹丽丽; 王全林
来源期刊	理化检验. B, 化学分册
ISSN	1001-4020
出版年	2014
卷号	50 期号:5 页码:606-610
中文摘要	采用超高效液相色谱-串联质谱法同时测定田螺中3种微囊藻毒素(微囊藻毒素-RR、微囊藻毒素-LR、微囊藻毒素-YR)的含量。田螺样品经甲醇-水(85+ 15)混合液提取,Oasis HLB固相萃取柱净化。以UPLCBEH C_(18)色谱柱为固定相,以不同体积比混合的(A)甲酸(0.1+ 99.9)溶液和(B)甲醇-乙腈(1+1)混合液为流动相做梯度洗脱,采用电喷雾正离子源模式多反应监测检测。3种微囊藻毒素的质量浓度均在1.0~100.0 mug?L~(-1)范围内与其峰面积呈线性关系,方法的检出限(3S/N)在0.05~0.08 mug?kg~(-1)之间。以空白样品为基体进行加标回收试验,所得回收率在67.3%~84.2%之间,相对标准偏差(n=6)在6.1%~8.3%之间。
英文摘要	UHPLC-MS/MS was applied to the simultaneous determination of 3 microcystic algae toxins (MC-RR, MC-LR and MC-YR) in field snail. The field snail sample was extracted with methanol (85 + 15) solution and purified by passing through Oasis HLB-solid phase extraction column. UPLC BEH C18 column was used as stationary phase,and mixtures of (A) formic acid (0. 1 + 99. 9) aq. solution and (B) mixture of methanol-acetonitrile (1 + 1) solution mixed in different ratios were used as mobile phase in gradient elution. ESI~+ as well as multi-reactions monitoring was adopted in MS/MS. Linear relationships between values of peak area and mass concentration of the 3 microcystic algae toxins were kept in the same range of 1. 0-100. 0 mug · L~(-1),with detection limits (3S/N) in the range of 0. 05 r 0. 08 pg · kg~(-1). On the base of blank sample, test for recovery was made by standard addition method; values of recovery found were in the range of 67. 3%-84. 2%,with RSD’s (n = 6) in the range of 6. 1%-8. 3%.
中文关键词	超高效液相色谱-串联质谱法 ; 田螺 ; 微囊藻毒素
英文关键词	UHPLC-MS/MS Field snail Microcystic algae toxins
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:5147902
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231428
作者单位	宁波市产品质量监督检验研究院, 宁波, 浙江 315041, 中国
推荐引用方式 GB/T 7714	沈坚,张爱芝,曹丽丽,等. 超高效液相色谱-串联质谱法同时测定田螺中3种微囊藻毒素[J],2014,50(5):606-610.
APA	沈坚,张爱芝,曹丽丽,&王全林.(2014).超高效液相色谱-串联质谱法同时测定田螺中3种微囊藻毒素.理化检验. B, 化学分册,50(5),606-610.
MLA	沈坚,et al."超高效液相色谱-串联质谱法同时测定田螺中3种微囊藻毒素".理化检验. B, 化学分册 50.5(2014):606-610.