干旱区生态环境知识资源中心(Arid): 固相萃取-液相色谱-串联质谱法测定猪栏废水中10种抗生素

Arid

	固相萃取-液相色谱-串联质谱法测定猪栏废水中10种抗生素
其他题名	LC-MS/MS Determination of 10 Antibiotics in Swinery Waste Water with Solid Phase Extraction
	闾幸 1; 余卫娟 2; 兰亚琼 3; 李立 3; 叶朝霞 2; 刘锐 3; 陈吕军 3; 张永明 1
来源期刊	理化检验. B, 化学分册
ISSN	1001-4020
出版年	2014
卷号	50 期号:3 页码:273-277
中文摘要	应用固相萃取-液相色谱-串联质谱法测定养猪废水中10种抗生素的含量。水样由盐酸调节pH至2. 5~4. 0,流经Oasis HLB固相萃取柱富集后再用甲醇洗脱。以Aglient eclipse XDBC_(18)色谱柱为分离柱,以不同体积比的0. 1%(体积分数)甲酸溶液和乙腈混合液为流动相进行梯度洗脱,采用电喷雾正离子源多反应监测模式检测。10种抗生素在一定的质量浓度范围内与其峰面积呈线性关系,方法的检出限(3S/N)在0. 03~3. 5 mug . L~(-1)之间。加标回收率在53. 2%~104%之间,测定值的相对标准偏差(n=6)在0. 5%~6. 3%之间。
英文摘要	LC-MS/MS was applied to the determination of 10 antibiotics in swinery waste water with solid phase extraction. The water sample was acidified to pH 2. 5 to 4. 0 with HCl, and enriched by passing through Oasis HLB SPE column. The column was eluted with methanol. For LC separation, aglient eclipse XDB C_(18) column was used as stationary phase, and the mixture of 0.1 % (phi) formic acid solution and acetonitrile mixed in different ratio was used as mobile phase in gradient elution. ESI~+ and multi-reaction monitoring were adopted in MS/MS. Linear relationships between values of peak area and mass concentration of the 10 antibiotics were kept in definite ranges. Detection limits (3S/N) found were ranged from 0. 03 to 3. 5 mug · L~(-1). Values of recovery found by standard addition method were in the range of 53. 2% -104%,with values of RSD’s (n=6) in the range of 0. 5%-6. 3%.
中文关键词	液相色谱-串联质谱法 ; 猪栏废水 ; 抗生素 ; 固相萃取
英文关键词	LC-MS/MS Swinery waste water Antibiotics Solid phase extraction
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:5094201
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231427
作者单位	1.上海师范大学生命与环境科学学院, 上海 200234, 中国; 2.嘉兴市环境保护监测站, 嘉兴, 浙江 314000, 中国; 3.浙江清华长三角研究院生态环境研究所, 嘉兴, 浙江 314006, 中国
推荐引用方式 GB/T 7714	闾幸,余卫娟,兰亚琼,等. 固相萃取-液相色谱-串联质谱法测定猪栏废水中10种抗生素[J],2014,50(3):273-277.
APA	闾幸.,余卫娟.,兰亚琼.,李立.,叶朝霞.,...&张永明.(2014).固相萃取-液相色谱-串联质谱法测定猪栏废水中10种抗生素.理化检验. B, 化学分册,50(3),273-277.
MLA	闾幸,et al."固相萃取-液相色谱-串联质谱法测定猪栏废水中10种抗生素".理化检验. B, 化学分册 50.3(2014):273-277.