干旱区生态环境知识资源中心(Arid): 血清中游离型双酚A的固相萃取-同位素内标-高效液相色谱串联质谱测定法

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	血清中游离型双酚A的固相萃取-同位素内标-高效液相色谱串联质谱测定法
其他题名	Determination of bisphenol A in serum by high-performance liquid chromatography-tandem mass spectrometry
	鲍慧惠 1; 盛杰 2; 许媛媛 1; 郝加虎 3; 吴皖珂 1; 苏普玉 3; 朱鹏 3; 陶芳标 3
来源期刊	环境与健康杂志
ISSN	1001-5914
出版年	2014
卷号	31 期号:1 页码:44-46
中文摘要	目的建立的测定孕妇血清中游离型双酚A的浓度固相萃取-同位素内标-高效液相色谱串联质谱(HPLC-MS/ MS)法。方法采用Oasis HLB固相萃取柱进行固相萃取,液相色谱的分离采用C_(18)色谱柱,使用乙腈和水作为流动相进行梯度洗脱。质谱运用负离子喷雾模式电离,多反应离子监测方式检测。结果该方法检测双酚A的线性范围为0.2?50 ng/ml,r=0.999 9,最低检测限为0.15ng/ml,定量限为0.4 ng/ml。0.5、2、10、50 ng/ml 4个浓度的加标回收率分别为114.8%, 108.1%, 100.8%,97.2%;日内分别为15.9%,9.3%,4.6%,3.5%;日间RSD分别为10.5%,15.1%,5.3%,5.1%。结论该方法测定孕妇血清中游离型双酚A具有较高的灵敏度、特异度和精确性,适用于血清样本中痕量双酚A的检测。
英文摘要	Objective To establish a method using isotope dilution-solid phase extraction (SPE) coupled to high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for the determination of free bisphenol A (BPA) concentration in human serum sample. Methods The Oasis HLB SPE column was used for the concentration and extraction of BPA in the serum. C_(18) chromatographic column was used with a gradient elution system of acetonitrile-water as the mobile phase, mass spectrometry under negative electrospray ionization mode and multiple reaction monitoring (MRM) were performed for the qualitative and quantitative analysis. Results The linear range was 0.2 -50 ng/ml, r =0.999 9. The limit of detection and quantification were 0.15 ng/ml and 0.4 ng/ml respectively. The spiked recoveries of BPA in serum samples of 0.5, 2, 10 and 50 ng/ml were 114.8%, 108.1%, 100.8% and 97.2%,respectively. Conclusion The method using isotope dilution -solid phase extraction (SPE) coupled to high-performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS) for the determination of free bisphenol A (BPA) concentration in human serum sample has high sensitivity, selectivity and precision, and is applicable to the measurement of trace level BPA in the serum.
中文关键词	双酚A ; 血清 ; 固相萃取 ; 高效液相色谱-串联质谱
英文关键词	Bisphenol A Serum Solid phase extraction (SPE) High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:5120290
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231372
作者单位	1.安徽医科大学公共卫生学院儿少卫生与妇幼保健学系, 合肥, 安徽 230032, 中国; 2.安徽医科大学公共卫生学院预防医学实验教学示范中心; 3.安徽医科大学公共卫生学院儿少卫生与妇幼保健学系, 安徽人口健康与优生省级实验室, 合肥, 安徽 230032, 中国
推荐引用方式 GB/T 7714	鲍慧惠,盛杰,许媛媛,等. 血清中游离型双酚A的固相萃取-同位素内标-高效液相色谱串联质谱测定法[J],2014,31(1):44-46.
APA	鲍慧惠.,盛杰.,许媛媛.,郝加虎.,吴皖珂.,...&陶芳标.(2014).血清中游离型双酚A的固相萃取-同位素内标-高效液相色谱串联质谱测定法.环境与健康杂志,31(1),44-46.
MLA	鲍慧惠,et al."血清中游离型双酚A的固相萃取-同位素内标-高效液相色谱串联质谱测定法".环境与健康杂志 31.1(2014):44-46.