干旱区生态环境知识资源中心(Arid): 高枝假木贼种子低温萌发与抗氧化酶系统的关系

Arid

	高枝假木贼种子低温萌发与抗氧化酶系统的关系
其他题名	Relationship between Low-temperature Germination and Antioxidant Enzyme Systems of Anabasis elatior Seeds
	李姝娟 1; 严成 1; 魏岩 2; 牟书勇 1
来源期刊	干旱区研究
ISSN	1001-4675
出版年	2014
卷号	31 期号:2 页码:302-306
中文摘要	高枝假木贼具有胎生萌发特性,并且发生在早春。为揭示该植物在早春低温环境中胎生萌发特性,在实验控制条件下,对种子吸胀萌发过程中3种保护酶活性和丙二醛(MDA)含量进行测定,并与野外自然条件下,不同发育阶段幼苗的保护酶活性变化进行对比分析。结果显示: 高枝假木贼种子吸水快,在室内5 ℃ 低温控制下6 h 吸水量趋于平稳。种子在低温吸胀过程中,MDA 的含量较高(0. 02 ~ 0. 04 mumol·g ~(- 1)),过氧化物酶(POD)活性保持在高水平稳定状态,过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性在吸胀4 h 之后快速上升。随着种子萌发,不同幼苗阶段 MDA 含量急剧下降,维持在0. 004 mumol·g ~(- 1)的极低水平,POD 活性显著下降,SOD 活性先下降后上升至幼苗3 cm 时达到最高,CAT 活性呈缓慢上升趋势。在野外低温条件下,SOD、CAT 和 POD 活性均显著高于5 ℃恒温控制下的幼苗酶活性水平。表明高枝假木贼快速吸胀萌发特性和3种保护酶在吸胀萌发不同阶段的协同作用,共同为胎生萌发在早春低温的正常生长提供保护。
英文摘要	Anabasis elatior is a viviparous subshrub that primarily occurs in the Junggar Desert. Vivipary occurs only in early spring. In order to understand how the physiological property of A. elatior adapts the low temperature, the MDA,antioxidant enzyme POD,CAT and SOD activities during imbibition and germination of A. elatior seeds were selected as the physiological indicators to study the physiological response under chilling stress. Moreover,a comparative study between laboratory control and natural environment was conducted to determine the change of activities of antioxidative enzyme POD,CAT and SOD during seed germination under chilling condition. The main results showed that the absolute absorbed water of seeds was stabilized at 5 ℃ after imbibition for 6 hours. MDA content of A. elatior seeds was maintained in a range from 0. 02 to 0. 04 mumol·g ~(- 1). A stable change of POD activity was remained. CAT and SOD activities were increased after 4 hours imbibition. Compared with the imbibition stage,MDA content of seedlings was declined sharply to a very low level (0. 002 - 0. 004 mumol·g ~(- 1)). POD activity was also significantly deceased. SOD activity was low at the early germination stage. Subsequently,SOD activity was gradually increased to the maximum. CAT activity was markedly increased at the first three growth stages (0 - 3 cm length) of seedlings,then decreased. The activities of antioxidant enzymes of seedlings in natural environment were higher than those at 5 ℃ in laboratory. The results revealed that both rapid water absorption and synergistic effects of three antioxidant enzymes provided a guarantee to form viviparous seedlings,and also a significance to revealing the propagation approach of desert viviparous shrubs.
中文关键词	高枝假木贼( Anabasis elatior) ; 种子 ; 低温 ; 吸胀萌发 ; 保护酶活性
英文关键词	Anabasis elatior seed low temperature imbibition and germination protecting enzyme activity
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENVIRONMENTAL SCIENCES
WOS研究方向	Environmental Sciences & Ecology
CSCD记录号	CSCD:5094310
来源机构	中国科学院新疆生态与地理研究所 ; 新疆农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231161
作者单位	1.中国科学院新疆生态与地理研究所, 乌鲁木齐, 新疆 830011, 中国; 2.新疆农业大学草业与环境科学学院, 乌鲁木齐, 新疆 830052, 中国
推荐引用方式 GB/T 7714	李姝娟,严成,魏岩,等. 高枝假木贼种子低温萌发与抗氧化酶系统的关系[J]. 中国科学院新疆生态与地理研究所, 新疆农业大学,2014,31(2):302-306.
APA	李姝娟,严成,魏岩,&牟书勇.(2014).高枝假木贼种子低温萌发与抗氧化酶系统的关系.干旱区研究,31(2),302-306.
MLA	李姝娟,et al."高枝假木贼种子低温萌发与抗氧化酶系统的关系".干旱区研究 31.2(2014):302-306.