干旱区生态环境知识资源中心(Arid): 超高效液相色谱-串联质谱法测定饲料中6大类50种药物

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	超高效液相色谱-串联质谱法测定饲料中6大类50种药物
其他题名	Simultaneous determination of 50 drugs in feeds by ultra performance liquid chromatography-electrospray ionization tandem quadrupole mass spectrometric
	赵健; 吕燕; 许秀琴; 吴银良; 杨挺
来源期刊	分析试验室
ISSN	1000-0720
出版年	2014
卷号	33 期号:6 页码:713-718
中文摘要	建立了饲料中超高效液相色谱-串联质谱法同时测定6类药物(磺胺类、喹诺酮类、氯霉素类、四环素类、大环内酯类和受体激动剂)。样品经甲醇/水/甲酸(体积比49/49/2)提取,HLB固相萃取小柱净化,超高效液相色谱-串联质谱仪测定, ESI正负离子同时扫描,以多反应监测方式采集数据,外标法定量。50种药物在饲料基质溶液中,在10~500 mug/L范围内具有良好的线性关系(r>0.99);在4个不同浓度加标水平下,各药物的平均回收率在60.4%~130%之间,RSD为1.1%~21%,方法的定量限为1~8 mug/kg。经实际饲料样品确证分析,方法适用于饲料中多种药物的同时检测。
英文摘要	An ultra performance liquid chromatography with electrospray ionization tandem mass spectrometric (UPLC-ESI-MS/MS) method was developed for the simultaneous determination of 50 drugs in feeds such as sulfonamides, chloramphenicols,quinolones, tetracyclines, macrolides and beta-agonists. The sample was extracted with methanol/water/formic acid (49/49/2, V/V/V), and then cleaned up using solid phase extraction with an Oasis HLB column, detected by UPLC-ESI-MS/MS. The ESI-MS/MS detection was achieved in positive and negative modes under multiple reaction monitoring (MRM) mode. The linear ranges were from 0.01 to 0.5 mg/L with the correlation coefficients above 0.99 for all the 50 drugs. The average recoveries of the 50 drugs spiked in feeds at four spiked levels were 60.4%~130% with the relative standard deviations of 1.1%~20.7%. The limits of detection were 1 mug/kg~8 mug/kg. The method is rapid, accurate, reproducible and sensitive, and suitable for the simultaneous determination of diverse drugs in compound feeds.
中文关键词	超高效液相色谱-串联质谱 ; 药物 ; 饲料
英文关键词	Ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry Drugs Feeds
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:5151239
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231041
作者单位	宁波市农业科学研究院, 宁波, 浙江 315101, 中国
推荐引用方式 GB/T 7714	赵健,吕燕,许秀琴,等. 超高效液相色谱-串联质谱法测定饲料中6大类50种药物[J],2014,33(6):713-718.
APA	赵健,吕燕,许秀琴,吴银良,&杨挺.(2014).超高效液相色谱-串联质谱法测定饲料中6大类50种药物.分析试验室,33(6),713-718.
MLA	赵健,et al."超高效液相色谱-串联质谱法测定饲料中6大类50种药物".分析试验室 33.6(2014):713-718.