干旱区生态环境知识资源中心(Arid): 高效液相色谱-串联质谱法测定辣椒及辣椒制品中金刚烷胺残留

Arid

	高效液相色谱-串联质谱法测定辣椒及辣椒制品中金刚烷胺残留
其他题名	Determination of Amantadine Residues in Pepper and Pepper Products by High Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry
	胡巧茹; 周长朋; 张玉春; 崔嘉; 鞠玲燕; 杨丽君
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2014
卷号	33 期号:10 页码:1184-1188
中文摘要	建立了高效液相色谱-串联质谱(HPLC-MS/MS)测定辣椒及辣椒制品中金刚烷胺残留的分析方法。样品采用1%三氯乙酸溶液-甲醇(1 : 1)提取,经Oasis-MCX固相萃取小柱净化后用Atlantis T3 C_(18)柱进行分离,以0.1%甲酸水-乙腈(85 : 15)作为流动相进行等度洗脱,电喷雾正离子(ESI +)扫描方式下多反应监测(MRM)模式对样品进行检测。实验结果表明,金刚烷胺在1.0~50 mug/kg范围内线性关系良好,鲜辣椒样品的定量下限为1.0 mug/kg,干辣椒及干辣椒制品为5.0 mug/kg。鲜辣椒在1.0,2.0,5. 0 mug/kg加标水平下的回收率为72.2%~98.2%,相对标准偏差(n=6)为1.2%~4.6% ;干辣椒及干辣椒制品在5. 0,10.0,25.0 mug/kg加标水平的回收率为64.2%~81.6%,相对标准偏差(n=6)为1.3%~6.3%。该方法操作简单、灵敏度高、重现性好,可用于辣椒及辣椒制品中金刚烷胺残留量的检测。
英文摘要	A high performance liquid chromatography tandem mass spectrometric (HPLC - MS/MS) method was developed for the determination of amantadine residues in pepper and pepper products. After extracted with 1 % trichloroacetic acid solution - methanol (1:1) and purified by an Oasis - MCX SPE cartridge, the samples were sepatated on an Atlantis T3 C_(18) column(3.0 mum, 2. 1 mm * 150 mm) by gradient elution. The electrospray was operated in positive mode and the samples were monitored under the multiple reaction monitoring(MRM) mode. The calibration curves showed good linearity in the range of 1. 0 -50 mug/kg. The quantitation limit was 1.0 mug/kg for fresh pepper, and 5. 0 mug/kg for dried pepper and chili products. The recoveries of amantadine in fresh pepper at spiked levels of 1.0, 2. 0, 5. 0 mug/kg were 72. 2% - 98. 2%, with relative standard deviations (RSDs, n =6) of 1. 2% -4. 6%, and recoveries in dry pepper and chili products at spiked levels at 5.0, 10.0, 25. 0 mug/kg were 64. 2% -81.6%, with RSDs (n=6) of 1. 3% -6. 3%. With advantages of simple operation, high sensitivity, good reproducibility, the method could be used for the detection of amantadine residues in the pepper and pepper products.
中文关键词	高效液相色谱-串联质谱 ; 金刚烷胺 ; 辣椒 ; 辣椒制品
英文关键词	high performance liquid chromatography - tandem mass spectrometry (HPLC - MS/MS) amantadine pepper pepper products
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL ; PHARMACOLOGY PHARMACY
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
CSCD记录号	CSCD:5266745
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231035
作者单位	威海出入境检验检疫局检验检疫技术中心, 威海, 山东 264205, 中国
推荐引用方式 GB/T 7714	胡巧茹,周长朋,张玉春,等. 高效液相色谱-串联质谱法测定辣椒及辣椒制品中金刚烷胺残留[J],2014,33(10):1184-1188.
APA	胡巧茹,周长朋,张玉春,崔嘉,鞠玲燕,&杨丽君.(2014).高效液相色谱-串联质谱法测定辣椒及辣椒制品中金刚烷胺残留.分析测试学报,33(10),1184-1188.
MLA	胡巧茹,et al."高效液相色谱-串联质谱法测定辣椒及辣椒制品中金刚烷胺残留".分析测试学报 33.10(2014):1184-1188.