干旱区生态环境知识资源中心(Arid): 基于线粒体COI和Cytb基因序列对新疆两水系斑重唇鱼遗传结构的分析

Arid

	基于线粒体COI和Cytb基因序列对新疆两水系斑重唇鱼遗传结构的分析
其他题名	Genetic structure analysis of Diptychus maculatus between two water systems in Xinjiang based on mitochondrial COI and Cyt b gene sequences
	杨天燕; 孟玮; 马燕武; 蔡林钢; 郭焱
来源期刊	淡水渔业
ISSN	1000-6907
出版年	2014
卷号	44 期号:4 页码:41-47
中文摘要	采用线粒体COI和Cyt b基因序列分析相结合的方法,对伊犁河和塔里木河水系6个斑重唇鱼(Diptychus maculates)群体遗传差异和分化水平进行了比较研究。 AMOVA分析结果显示,斑重唇鱼群体间遗传差异显著大于群体内,群体平均遗传分化指数PhiST分别为0.9802(COI)和0.8863(Cyt b),除伊犁河水系两群体差异不显著外(P>0.05),其他两两群体间遗传差异均显著。基于Kimura双参数法计算群体间平均遗传距离发现,不仅两水系间群体存在差异,塔里木河水系内部群体也产生了较大的遗传分化。单倍型网络图和系统关系树显示库玛拉克河群体存在两组不同单倍型类群,分别与伊犁河和塔里木河水系单倍型聚类。根据分子钟假说,基于Cyt b基因序列推算斑重唇鱼两支系发生分歧的时间大约在2.41 Ma年的上新世末到更新世初。天山第四纪构造运动第一幕可能是两水系斑重唇鱼发生遗传分化的重要原因,而塔里木河水系群体发生遗传分化可能与塔克拉玛干沙漠的阻隔有关。
英文摘要	In this study, the mitochondrial COI and Cyt b gene sequences were analyzed to reveal the genetic diversity and divergence level in six Diptychus maculatus populations. AMOVA analysis showed that genetic variation among populations was significantly greater than that within populations, and the average genetic differentiation index PhiST were 0.980 2 (COI) and 0.886 3 (Cyt b) respectively.Genetic differences between two populations were significant except two populations from Ili River (P>0.05).Average genetic distance based on Kimura two-parameter suggested that not only populations between two river systems showed significant genetic differentiations but certain populations among Tarim River also showed differentiations.Haplotype network charts and phylogenetic trees both revealed Kumalake River population existed two different haplogroups.According to the molecular clock hypothesis, phylogenetic divergence time was about 2.41 Ma when it was late Pliocene to early Pleistocene.The Quaternary tectonic movement in the first act of Tianshan Mountain might be the most important reason for genetic divergence in D.maculates populations from two water systems.However, the genetic divergence in D.maculates populations from Tarim River might be caused by the separation of Taklimakan Desert.
中文关键词	斑重唇鱼(Diptychus maculates) ; 伊犁河 ; 塔里木河 ; COI基因 ; Cyt b基因 ; 遗传结构
英文关键词	Diptychus maculatus Ili River Tarim River COI gene Cyt b gene genetic structure
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FISHERIES
WOS研究方向	Fisheries
CSCD记录号	CSCD:5222471
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/230900
作者单位	新疆维吾尔自治区水产科学研究所, 乌鲁木齐, 新疆 830000, 中国
推荐引用方式 GB/T 7714	杨天燕,孟玮,马燕武,等. 基于线粒体COI和Cytb基因序列对新疆两水系斑重唇鱼遗传结构的分析[J],2014,44(4):41-47.
APA	杨天燕,孟玮,马燕武,蔡林钢,&郭焱.(2014).基于线粒体COI和Cytb基因序列对新疆两水系斑重唇鱼遗传结构的分析.淡水渔业,44(4),41-47.
MLA	杨天燕,et al."基于线粒体COI和Cytb基因序列对新疆两水系斑重唇鱼遗传结构的分析".淡水渔业 44.4(2014):41-47.