干旱区生态环境知识资源中心(Arid): 全血中17种农药同时测定的固相萃取气相色谱质谱法

Arid

	全血中17种农药同时测定的固相萃取气相色谱质谱法
其他题名	Determination method for 17 pesticides in common in whole blood by gas chromatogvaphy coupled to mass spectrometry with solid-phase extraction sample cleanup
	周博; 李惠玲; 马婧; 董芳; 郝凤桐
来源期刊	中华劳动卫生职业病杂志
ISSN	1001-9391
出版年	2013
卷号	31 期号:9 页码:709-712
中文摘要	目的建立同时检测全血中17种常见农药的固相萃取净化气相色谱-质谱(SPE-GC-MS)检测方法。方法全血样品用乙腈提取,Oasis HLB固相萃取柱净化,用气相色谱进行分离,选择离子扫描监测(SIM)的质谱扫描模式下定量分析。结果全血中17种农药添加浓度在1.0~5.0mg/L之间,加标回收率为41.3%~102.1%,大部分农药的相对标准偏差<10%,17种农药在0.5-5.0mg/L浓度范围线性关系良好,相关系数为0.9945~0.9994,定量检出限为0.05~0.09mg/L。结论该方法能够将全血中17种农药进行良好的分离测定,有较高的灵敏度、准确度和精密度,可满足中毒血样中多种农药的定性及定量检测分析的要求。
英文摘要	Objective To establish a method for simultaneous determination of 17 common pesticides in whole blood by solid phase extraction-gas chromatography-mass spectrometry (SPE-GC-MS). Methods Whole blood samples were treated by extraction with acetonitrile, and the obtained extract was cleaned up using an Oasis HLB SPE cartridge; pesticides were separated by GC and quantitatively analyzed by MS with selected ion monitoring. Results The concentrations of 17 pesticides in whole blood were 1.0-5.0 mg/L, and the recovery rate was 41.3-102.1%, with a relative standard deviation of less than 10% in most pesticides. The 17 pesticides showed a good linear relationship between concentration and peak area within 0.5-5.0 mg/L, with a correlation coefficient of 0.9945-0.9994. The limit of detection and limit of quantification were 0.02-0.05 mg/L and 0.05-0.09 mg/L, respectively. Conclusion With this method, 17 pesticides in whole blood can be well separated and determined. This method has high sensitivity, accuracy, and precision and can be used for identification and quantification of multiple pesticides in blood samples.
中文关键词	血液 ; 农药 ; 色谱法 ; 气相
英文关键词	Blood Pesticides Chromatography gas
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PUBLIC ENVIRONMENTAL OCCUPATIONAL HEALTH
WOS研究方向	Public, Environmental & Occupational Health
CSCD记录号	CSCD:4934656
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/230730
作者单位	首都医科大学附属北京朝阳医院职业病与中毒医学科, 北京 100020, 中国
推荐引用方式 GB/T 7714	周博,李惠玲,马婧,等. 全血中17种农药同时测定的固相萃取气相色谱质谱法[J],2013,31(9):709-712.
APA	周博,李惠玲,马婧,董芳,&郝凤桐.(2013).全血中17种农药同时测定的固相萃取气相色谱质谱法.中华劳动卫生职业病杂志,31(9),709-712.
MLA	周博,et al."全血中17种农药同时测定的固相萃取气相色谱质谱法".中华劳动卫生职业病杂志 31.9(2013):709-712.