干旱区生态环境知识资源中心(Arid): SPE-HPLC-DAD同时测定大鼠血浆中斯皮诺素和6′′′-阿魏酰斯皮诺素的含量

Arid

	SPE-HPLC-DAD同时测定大鼠血浆中斯皮诺素和6′′′-阿魏酰斯皮诺素的含量
其他题名	Determination of Spinosin and 6′′′-Feruloylspinosin in Rat Plasma after Oral Administration of Flavonoid Extract from Ziziphi Spinosae Semen by SPE-HPLC-DAD
	鲍康德 1; 赵继红 2; 齐炼文 3
来源期刊	中国现代应用药学
ISSN	1007-7693
出版年	2013
卷号	30 期号:7 页码:707-711
中文摘要	目的建立一种便捷、灵敏定量分析酸枣仁黄酮提取物在大鼠血浆中代谢成分的方法。方法大鼠口服酸枣仁黄酮提取物后于0~24 h内分别自各组大鼠眼眶后窦采血,血样经固相萃取预处理后采用高效液相-二极管阵列检测器联用色谱法(SPE-HPLC-DAD)定量分析其主要代谢成分。结果酸枣仁黄酮提取物在血浆中主要代谢成分仍以原形形式存在;斯皮诺素和6′′′-阿魏酰斯皮诺素在血浆中的固相萃取平均回收率分别为84.76%和84.33%;斯皮诺素在0.52~119.31mg?L~(-1)(r>0.999 8)、6′′′-阿魏酰斯皮诺素在0.38~91.73 mg?L~(-1)(r>0.999 7)内线性关系良好,二者平均血浆药物浓度于给药后的6 h和4 h达峰值,并在体内缓慢代谢完毕。结论 SPE-HPLC-DAD可用于定量分析酸枣仁黄酮提取物在大鼠体内的代谢过程,为中药主要活性成分在生物体内药动学特征研究提供了一种有效、快捷途径。
英文摘要	OBJECTIVE To develop a convenient and sensitive method for determination of flavonoid metabolites in rat plasma after oral administration of Ziziphi Spinosae Semen extract. METHODS The rat blood samples were collected from each rat by puncture of the retro-orbital sinus from 0 to 24 h after oral administration of flavonoid extract of jujube seeds, then pretreated with a Waters OASIS~(TM) SPE column using cartridges. The plasma samples were analyzed by HPLC-DAD method to detect the main metabolites. RESULTS The original flavonoids were the major forms of flavonoid metabolites in rat plasma. The calibration curves were linear over the range of 0.52-119.3 mug·mL~(-1)(r>0.999 8) for spinosin and 0.38-91.7 mug·mL~(-1) (r>0.999 7) for 6′′′-feruloylspinosin with the mean extraction recoveries as 84.76% and 84.33%, respectively. The average plasma drug concentration of them reached the top level at the 12th and 24th hour after oral administration, and disappeared gradually in vivo. CONCLUSION This convenient SPE-HPLC-DAD method can be used to determinate the flavonoid metabolites of Ziziphi Spinosae Semen. The pharmacokinetic feature of main active components of traditional Chinese medicine in vivo can be characterized as well.
中文关键词	酸枣仁黄酮提取物 ; 血浆 ; 代谢物 ; 固相萃取-高效液相色谱-二极管阵列检测法
英文关键词	flavonoid extract of Ziziphi Spinosae Semen plasma metabolite SPE-HPLC-DAD
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PHARMACOLOGY PHARMACY
WOS研究方向	Pharmacology & Pharmacy
CSCD记录号	CSCD:4962325
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/230713
作者单位	1.浙江康莱特药业有限公司药研所, 杭州, 浙江 310018, 中国; 2.浙江康莱特新森医药原料有限公司质管部, 杭州, 浙江 310018, 中国; 3.中国药科大学, 天然药物国家重点实验室, 南京, 江苏 210009, 中国
推荐引用方式 GB/T 7714	鲍康德,赵继红,齐炼文. SPE-HPLC-DAD同时测定大鼠血浆中斯皮诺素和6′′′-阿魏酰斯皮诺素的含量[J],2013,30(7):707-711.
APA	鲍康德,赵继红,&齐炼文.(2013).SPE-HPLC-DAD同时测定大鼠血浆中斯皮诺素和6′′′-阿魏酰斯皮诺素的含量.中国现代应用药学,30(7),707-711.
MLA	鲍康德,et al."SPE-HPLC-DAD同时测定大鼠血浆中斯皮诺素和6′′′-阿魏酰斯皮诺素的含量".中国现代应用药学 30.7(2013):707-711.