干旱区生态环境知识资源中心(Arid): 高效液相色谱-串联质谱法测定鸡蛋中癸氧喹酯的残留

Arid

	高效液相色谱-串联质谱法测定鸡蛋中癸氧喹酯的残留
其他题名	Residue Determination of Decoquinate in Eggs by High Performance Liquid Chromatography-tandem Mass Spectrometry
	王中艳 1; 罗浩师 1; 张丽芳 1; 江善祥 2; 费陈忠 1; 张可煜 1; 郑文丽 1; 王霄旸 1; 薛飞群 1
来源期刊	中国农学通报
ISSN	1000-6850
出版年	2013
卷号	29 期号:8 页码:21-25
中文摘要	为建立一种快速、灵敏的高效液相色谱-串联质谱法(LC-MS/MS),以应用于癸氧喹酯(DEC)在鸡蛋中的残留检测,并将该方法用于残留消除规律的研究。将8只健康蛋鸡连续饲喂添加癸氧喹酯(浓度为27.2mg/kg)的饲料10天后,分别在停药后第1、2、3、5、8、11天收集鸡蛋匀浆,用乙腈:乙酸乙酯(1:1)提取,Oasis~(TM)HLB固相萃取柱净化,以0.1%甲酸水:乙腈(30/70,v/v)作为流动相,XTerraC_(18)色谱柱洗脱,质谱多反应监测(MRM)模式进行检测。结果表明:该方法的灵敏度高,检测限(LOD)和定量限(LOQ)分别为0.2、0.8mug/kg,DEC在0.8~240mug/kg浓度范围内线性良好,相关系数R~2>0.993,回收率在90.7%~101.8%之间,RSD<11.4%;消除试验结果表明:鸡蛋中DEC最高残留浓度出现在停药后第3天,随着时间的推移,鸡蛋中癸氧喹酯的残留浓度逐渐降低,到第8天时该药的残留浓度达到欧洲委员会规定的允许浓度水平(20mug/kg)。本方法简便可靠,可满足检验实际需求。
英文摘要	A rapid, sensitive and reliable high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the analysis of DEC in eggs, and the method has been successfully applied to the depletion study of DEC. The eight healthy laying hens fed medicated feed with decoquinate concentration of 27.2 mg/kg for 10 consecutive days. The egg samples were collected on days 1, 2, 3, 5, 8, 11 after the last dose and homogenized. The compound was extracted by acetonitrile-ethyl acetate using liquid-liquid extraction (LLE), and purified with Oasis~(TM) HLB Solid Phase extraction cartridge. Chromatographic separation was performed on an XTerra C_(18) reversed-phase column with a mobile-phase of water containing 0.1% formic acid and acetonitrile. The analyte was detected by tandem quadrupole mass spectrometry after positive electrospray ionization by multiple reaction monitoring (MRM). The results demonstrated that the method has high sensitivity with the LOD of 0.2 mug/kg and the LOQ of 0.8 mug/kg. The calibration curve showed good linearity with the concentrations of 0.8-240 mug/kg with the correlation coefficient (R~2>0.993). The recovery ranges from 90.7% to 101.8%, andRSD <11.4%. The result of depletion profile of DEC showed that the highest residue concentration of DEC was attained 3 days post-treatment. The residue concentration of DEC decreased to 20 mug/kg which was the allowable concentration level of European Commission at 8 days after the last dose. The result showed that the method was rapid and reliable for application in practice.
中文关键词	癸氧喹酯 ; 高效液相色谱-串联质谱 ; 鸡蛋 ; 残留消除
英文关键词	Decoquinate HPLC-MS/MS eggs residue depletion
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:4891708
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/230503
作者单位	1.中国农业科学院上海兽医研究所/中国农业科学院兽药安全评价与兽药残留重点开放实验室, 农业部动物寄生虫病学重点实验室, 上海 200241, 中国; 2.南京农业大学动物医学院/兽医药理与毒理实验室, 南京, 江苏 210095, 中国
推荐引用方式 GB/T 7714	王中艳,罗浩师,张丽芳,等. 高效液相色谱-串联质谱法测定鸡蛋中癸氧喹酯的残留[J],2013,29(8):21-25.
APA	王中艳.,罗浩师.,张丽芳.,江善祥.,费陈忠.,...&薛飞群.(2013).高效液相色谱-串联质谱法测定鸡蛋中癸氧喹酯的残留.中国农学通报,29(8),21-25.
MLA	王中艳,et al."高效液相色谱-串联质谱法测定鸡蛋中癸氧喹酯的残留".中国农学通报 29.8(2013):21-25.