干旱区生态环境知识资源中心(Arid): 超高效液相色谱-质谱法测定全血中甲哌啶

Arid

	超高效液相色谱-质谱法测定全血中甲哌啶
其他题名	Determination of mepiquat chloride in whole blood with ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)
	赵森 1; 张云峰 2; 王炯 2; 于忠山 2
来源期刊	中国法医学杂志
ISSN	1001-5728
出版年	2013
卷号	28 期号:5 页码:406-408,416
中文摘要	目的建立了超高效液相色谱串联质谱法(UPLC-MS/MS)测定全血中甲哌啶的方法。方法样品血经pH=8氨水稀释后,涡旋离心,上清液过混合型弱阳离子交换柱(Oasis?WCX)进行纯化,采用UPLC-MS/MS检测。质谱检测采用正离子扫描,多反应离子监测模式(MRM)。以甲哌啶母离子114.098(m/z)和子离子58.078(m/z)及98.215(m/z)定性、定量。结果加标回收率在65%~75%。在S/N≥3的情况下,检出限为0.3ng/mL。结论实验结果证明该方法灵敏度高,分析速度快,操作简便,适宜应用于法庭科学领域中解决甲哌啶中毒案件的检测。
英文摘要	Objective A UPLC-MS/MS method is proposed for the quantitative determination and confirmation of mepiquat chloride. Methods The whole blood was diluted by water, then vortexed and centrifuged. The supernatant was cleaned-up using Oasis? WCX solid phase extraction cartridges. Etracts were analyzed by UPLC-MS/MS in positive eletrospray ionization and multiple reaction monitoring (MRM) mode. The parent ion for difenzoquat is m/z 114. 098. Two daughter ions were monitored at m/z 58.078 and 98. 215 for quantification and confirmation. Results The average recoverys of mepiquat chloride from the whole blood samples were 65% ~ 75%. The detection limit was 0.33ng/mL. Conclusion The method was proved to be selective, sensitive and rapid.
中文关键词	法医毒物分析 ; 超高效液相色谱串联质谱法 ; 甲哌啶 ; 血液
英文关键词	forensic toxicological analysis UPLC-MS/MS mepiquat chloride blood
语种	中文
国家	中国
收录类别	CSCD
WOS类目	POLITICAL SCIENCE
WOS研究方向	Government & Law
CSCD记录号	CSCD:4977967
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/230480
作者单位	1.浙江警察学院, 杭州, 浙江 310053, 中国; 2.公安部物证鉴定中心, 北京 100038, 中国
推荐引用方式 GB/T 7714	赵森,张云峰,王炯,等. 超高效液相色谱-质谱法测定全血中甲哌啶[J],2013,28(5):406-408,416.
APA	赵森,张云峰,王炯,&于忠山.(2013).超高效液相色谱-质谱法测定全血中甲哌啶.中国法医学杂志,28(5),406-408,416.
MLA	赵森,et al."超高效液相色谱-质谱法测定全血中甲哌啶".中国法医学杂志 28.5(2013):406-408,416.