干旱区生态环境知识资源中心(Arid): 固相萃取-气相色谱法检测全血中佐匹克隆

Arid

	固相萃取-气相色谱法检测全血中佐匹克隆
其他题名	Determination of zopiclone in human blood by solid-phase extraction gas-chromatography
	舒翠霞 1; 张蕾萍 2; 董颖 2; 王姝姗 1; 牛晓东 1; 刘俊亭 1
来源期刊	中国法医学杂志
ISSN	1001-5728
出版年	2013
卷号	28 期号:1 页码:46-48
中文摘要	目的采用固相萃取-气相色谱法检测全血中佐匹克隆。方法采用Oasis HLB固相萃取柱对样品进行前处理,去离子水、0.5%氨水-甲醇/水((V/V 40∶60)溶液先后淋洗,二氯甲烷/异丙醇(V/V 75∶25)洗脱后进行GC/NPD检测。结果血液中佐匹克隆在50~5 000ng/mL范围内线性良好(R2=0.998 8),平均萃取回收率为96.9%,检出限为30ng/mL,日内RSD为2.1%~5.7%,日间RSD为3.3%~6.2%,结论固相萃取-气相色谱检测法灵敏度高,重现性好,可在血液中佐匹克隆的检测中选用。
英文摘要	Objective To establish a method for determination of zopiclone in human plasma by solidphase extraction gas-chromatography. Methods We extracted zopiclone from plasma by solid-phase extraction (OASIS HLB 1mL cartridge) and detected it with GC /NPD. The SPE column was rinsed sequentially with deionized water and 0. 5% ammonia in methanol-water(V/V 40∶60),and then eluted with dichloromethanepropanol(V/V 75 ∶ 25). Results The linear range of zopiclone was 50 ~ 5 000ng /mL(R2 = 0. 998 8,the average extraction recoveries were > 96. 9% and the lower limit of quantitation was 50ng /mL,the inter-day and intra-day precisions varied from 2. 1% to 5. 7% and from 3. 3% to 6. 2%,respectively. Conclusion The method has high sensitivity and good reproducibility without the endogenous interference. The result is accurate and reliable with a wide linear range. It can be used in forensic toxicological analysis.
中文关键词	法医毒物分析 ; 气相色谱 ; 固相萃取 ; 佐匹克隆
英文关键词	forensic toxicological analysis gas chromatography solid-phase extraction zopiclone
语种	中文
国家	中国
收录类别	CSCD
WOS类目	POLITICAL SCIENCE
WOS研究方向	Government & Law
CSCD记录号	CSCD:4791795
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/230479
作者单位	1.中国医科大学药学院药物分析教研室, 沈阳, 辽宁 110001, 中国; 2.公安部物证鉴定中心, 北京 100038, 中国
推荐引用方式 GB/T 7714	舒翠霞,张蕾萍,董颖,等. 固相萃取-气相色谱法检测全血中佐匹克隆[J],2013,28(1):46-48.
APA	舒翠霞,张蕾萍,董颖,王姝姗,牛晓东,&刘俊亭.(2013).固相萃取-气相色谱法检测全血中佐匹克隆.中国法医学杂志,28(1),46-48.
MLA	舒翠霞,et al."固相萃取-气相色谱法检测全血中佐匹克隆".中国法医学杂志 28.1(2013):46-48.