干旱区生态环境知识资源中心(Arid): 超高效液相色谱-串联质谱法同时测定化妆品中21种糖皮质激素

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	超高效液相色谱-串联质谱法同时测定化妆品中21种糖皮质激素
其他题名	UPLC -MS /MS determination of 21 glucocorticoids in cosmetics
	王伟萍; 张明玥; 蔺娟; 帕孜来提·亚库甫
来源期刊	药物分析杂志
ISSN	0254-1793
出版年	2013
卷号	33 期号:5 页码:837-843
中文摘要	目的:建立快速、准确、灵敏检测化妆品中添加的21种糖皮质激素:曲安西龙、波尼松龙、氢化可的松、波尼松、可的松、倍他米松、地塞米松、氟米松、倍氟米松、波尼松龙醋酸酯、氢化可的松醋酸酯、氟米龙、地夫可特、氟氢可的松醋酸酯、甲基泼尼松龙醋酸酯、布地奈德、泼尼卡酯、安西奈德、哈西奈德、倍氟米松双丙酸酯、地塞米松醋酸酯的分析方法。方法:样品用饱和氯化钠溶液分散,用乙腈从分散液中提取激素类药物,用亚铁氰化钾和醋酸锌从提取液中沉淀大分子基质,经Oasis HLB固相萃取小柱富集净化;采用SHIMADZU Shim-pack XR-ODS色谱柱(3.0 mm*75 mm,1.8mum),水(含0.1%乙酸)(A)-乙腈(含0.1%乙酸)(B)梯度洗脱,流速0.2 mL.min~(-1);离子源为ESI源,正离子扫描模式,多级反应监测测定。结果:该法各组分的平均回收率为85.7%~100.0%,RSD为2.6%~8.9%,方法检出限为0.005~0.053mug.g~(-1)。结论:该法能满足检测化妆品中21种糖皮质激素的需要。
英文摘要	Objective: To establish a quick,accurate, sensitive method for analysis of 21 glucocorticoids (triamcinolone, prednisolone,hydrocortisone,prednisone,cortisone,betamethasone,dexamethasone, flumethasone,beclomethasone, prednisolone, 21 - acetate, fluoromethalone,hydrecortisone 21 - acetateby, deflazacort, fludrocortisone 21 - acetate, methylprednisolone 21 - cetate,budesonide,dexamethasone - 21acetate,prednicarbate,halcinonide,amcinonide, beclometasone dipropionate) in cosmetics by UPLC - MS /MS. Methods: Sample was dispersed with saturated sodium chloride solution,extracted by acetonitrile scattered; then macromolecules from matrix were precipitated with ferrocyanatum kalium and zinc acetate,and finally extracted,enriched and purified by Oasis HLB macromolecular matrix solid - phase extraction and columella. The chromatographic was performed using a SHIMADZU Shim - pack XR - ODS analysis column(3. 0 mm * 75 mm,1. 8 mum) with gradient elution of water(including 0. 1% acetic acid) (A) - acetonitrile(including 0. 1% acetic acid) (B) at a flow rate of 0. 2 mL·min ~(- 1); ESI source was applied and operated in positive ion mode and multiple reaction monitoring (MRM). Results: The recoveries were 85. 7% - 100. 0%,and the RSDs were 2. 6% - 8. 9%; The method detection limits were 0. 005 - 0. 053 mug·g ~(- 1). Conclusion: The method can be used to detect 21 glucocorticoids in cosmetics.
中文关键词	超高效液质联用法 ; 固相萃取小柱 ; 化妆品安全监测 ; 糖皮质激素 ; 多级反应监测
英文关键词	UPLC - MS /MS solid phase extraction small column cosmetics safety monitoring glucocorticoids multiple reaction monitoring(MRM)
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PHARMACOLOGY PHARMACY
WOS研究方向	Pharmacology & Pharmacy
CSCD记录号	CSCD:4843400
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/230392
作者单位	新疆维吾尔自治区食品药品检验所, 乌鲁木齐, 新疆 830004, 中国
推荐引用方式 GB/T 7714	王伟萍,张明玥,蔺娟,等. 超高效液相色谱-串联质谱法同时测定化妆品中21种糖皮质激素[J],2013,33(5):837-843.
APA	王伟萍,张明玥,蔺娟,&帕孜来提·亚库甫.(2013).超高效液相色谱-串联质谱法同时测定化妆品中21种糖皮质激素.药物分析杂志,33(5),837-843.
MLA	王伟萍,et al."超高效液相色谱-串联质谱法同时测定化妆品中21种糖皮质激素".药物分析杂志 33.5(2013):837-843.