Arid
石漠化地区桑根际AM真菌多样性及桑壮苗培育研究
其他题名Diversity of AMF in Mulberry Rhizosphere in a Rock Desertification Area and Vigorous Mulberry Seedling Culture
施松梅1; 陈珂1; 涂波1; 杨晓红1; 黄先智2
来源期刊西南大学学报. 自然科学版
ISSN1673-9868
出版年2013
卷号35期号:10页码:24-30
中文摘要石漠化地区桑树根际AM真菌具有多样性,鉴定了3属(球囊霉属、无梗囊霉属和巨孢囊霉属)16种AM真菌,其中球囊霉属和无梗囊霉属为石漠化地区AM真菌优势属.以AM真菌和桑苗为试材,网室试验池中分别对生长2个月的无菌根桑苗接种摩西球囊霉、根内球囊霉和细凹无根囊霉3个AM真菌菌种,45d后测量接种和不接种桑苗的菌根侵染率、菌根依赖性、生长量和光合特性等指标.结果表明:网室中接种45 d的桑苗菌根侵染率最高达到(38. 671. 44) %,桑苗对真菌菌根依赖性最高达到(203. 0314. 93) %,接种菌根桑苗株高、茎粗、叶片数、第五片叶大小、主根长、植株鲜质量、干质量等都显著高于对照,菌根桑苗的叶绿素含量、光合速率、蒸腾速率、气孔导度显著高于非菌根桑苗.研究结果证明:桑苗对菌根真菌有着高度的依赖性,提高根系吸收能力,促进叶片光合作用,接种AM真菌能显著地促进桑苗根系和枝叶的生长,获得健壮的菌根化桑苗.
英文摘要The rhizosphere of mulberry in the rocky desertification regions has diverse arbuscular mycorrhiza fungi (AMF). In an investigation reported in this paper, three genera of AMF (Glomus, Acaulospora and Gigarspora) with sixteen species were identified according to their morphological characteristics, of which Acaulospora and Glomus were dominant genera around the rhizosphere of mulberry. Virus-free mulberry seedlings which had grown in experimental ponds in a netted chamber for 2 mouths were inoculated with Glomus mosseae, Glomus intraradices and Acaulospora scrobiculata separately, and mycorrhizal colonization rate, mycorrhizal dependency, growth and photosynthetic characteristics of the inoculated and uninoculated seedlings were measured after cnoculation for 45 day. The results showed the most colonization rate of mulberry roots was (38. 67 1. 44) % and the most mycorrhizal dependency of the mulberry seedlings was (203. 03 14. 93) %. The inoculated mulberry seedlings grew significantly better than the uninoculated control in the respects of seedlings height, taproot length, leaves per plant, area of the fifth leaf, fresh weight and dry weight of the plant. The content of chlorophyll net photosynthetic rate, transpiration rate, stomatal conductance of AMF-inoculated mulberry were significantly higher than those of non-AMF-inoculated plants. The above research results confirmed that the inoculants mulberry was highly dependent on AMF. AMF significantly enhanced the root absorption capacity, improved leaf photosynthesis and promoted the growth and development of the seedlings. A lot of vigorous seedlings of mulberry mycorrhizaed were harvested in the experimental ponds.
中文关键词石漠化 ; 多样性 ; 桑树 ; 壮苗
英文关键词AMF rocky desertification AMF diversity mulberry vigorous seedling
语种中文
国家中国
收录类别CSCD
WOS类目AGRICULTURE MULTIDISCIPLINARY
WOS研究方向Agriculture
CSCD记录号CSCD:4975303
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/230333
作者单位1.西南大学园艺园林学院, 南方山地园艺学教育部重点实验室, 重庆 400716, 中国;
2.西南大学蚕学与系统生物字研究所, 重庆 400716, 中国
推荐引用方式
GB/T 7714
施松梅,陈珂,涂波,等. 石漠化地区桑根际AM真菌多样性及桑壮苗培育研究[J],2013,35(10):24-30.
APA 施松梅,陈珂,涂波,杨晓红,&黄先智.(2013).石漠化地区桑根际AM真菌多样性及桑壮苗培育研究.西南大学学报. 自然科学版,35(10),24-30.
MLA 施松梅,et al."石漠化地区桑根际AM真菌多样性及桑壮苗培育研究".西南大学学报. 自然科学版 35.10(2013):24-30.
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