干旱区生态环境知识资源中心(Arid): 新疆荒漠盐碱环境中抗动物病原菌的放线菌筛选与鉴定

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	新疆荒漠盐碱环境中抗动物病原菌的放线菌筛选与鉴定
其他题名	Screening and identification of antagonistic actinomycetes against animal pathogen from saline-alkali soil in desert of Xinjiang
	马玥 1; 来航线 1; 韦小敏 1; 王海利 2; 张珊珊 1; 张艳群 1
来源期刊	西北农林科技大学学报. 自然科学版
ISSN	1671-9387
出版年	2013
卷号	41 期号:3 页码:133-140
中文摘要	【目的】从新疆荒漠地区特殊生境中,分离筛选对动物病原菌具有较高抑制活性的放线菌。【方法】采用皿内琼脂块法和发酵液滤纸片法,对新疆荒漠盐碱土环境中分离到的412株放线菌的拮抗性进行筛选,并对其中拮抗活性高的菌株进行形态特征、培养特征、生理生化特性及16S rRNA序列的分析鉴定,初步确定其分类地位。【结果】筛选结果显示:新疆地区荒漠土中39.6%的放线菌对所选的8种病原菌有拮抗作用,且随着靶标菌数的增加,拮抗比例减小;供试菌对革兰氏阳性菌的拮抗效果优于革兰氏阴性菌,对牛无乳链球菌Ⅰ、Ⅱ和猪金黄色葡萄球菌Ⅰ、Ⅱ的抑菌效果平均为26.0%和22.3%。通过初筛和复筛得到10株抑菌效果较好的拮抗性放线菌,经多相分类鉴定确定为链霉菌属的不同种,具体分类地位如下:XJ 6-2菌株为天蓝褐链霉菌(Streptomyces coeruleofuscus),XJ 1-5-10菌株为粪生链霉菌(Streptomyces fimicarius),XJ 2-9菌株为灰平链霉菌(Streptomyces griseoplanus),XJ 2-5-1菌株为弗氏链霉菌(Streptomyces fradiae),XJ 19-11菌株为变异链霉菌(Streptomyces variabilis),XJ 1202菌株为苍黄链霉菌(Streptomyces luridus),XJ 1625菌株为微黄绿链霉菌(Streptomyces flavidovirens),XJ 2-9-7菌株为库尔萨诺夫链霉菌(Streptomyces kurssanovii),XJ 617菌株为堆孢链霉菌(Streptomyces massasporeus),而XJ 1-6-5菌株的序列相似度在98%以下,可能为潜在新种,需要进一步鉴定。【结论】新疆地区荒漠土中存在大量拮抗性放线菌,并且获得了10株对动物病原菌有较高抑制活性的放线菌。
英文摘要	【Objective】 This study aimed to obtain actinomycetes with antibacterial spectrum and high activity against animal pathogen from special habitats in Xinjiang.【Method】 412 actinomycetes isolated from saline soil in typical desert areas of Xinjiang Province screened by agar block and fermentation liquor experiment.Then,the morphological,cultural,physiological and biochemical characteristics and 16S rRNA sequence analysis of selected high antimicrobial actinomycetes were identified.【Result】 39.6% actinomycetes showed inhibiting activity to 8 animal pathogens.With the increase of number of target bacteria the antagonistic ratios gradually decreased.These actinomycetes exhibited a higher inhibit activity against the gram-positive bacteria compared to the gram-negative bacteria.26.0% and 22.3% of the antagonistic actinomycetes showed antagonism against bovine Streptococcus agalactiae and swine Staphalococcus aureus,respectively.Finally,10 excellent antagonistic strains were obtained and identified by polyphasic taxonomy:XJ 6-2 is Streptomyces coeruleofuscus,XJ 1-5-10 is Streptomyces fimicarius,XJ 2-9 is Streptomyces griseoplanus,XJ 2-5-1 is Streptomyces fradiae,XJ 19-11 is Streptomyces variabilis,XJ 1202 is Streptomyces luridus,XJ 1625 is Streptomyces flavidovirens,XJ 2-9-7 is Streptomyces kurssanovii,and XJ 617 is Streptomyces massasporeus,The sequence similarity of XJ 1-6-5 was below 98.0%,it may be a potential new species and need further evaluation.【Conclusion】 There existed a lot of soil antagonistic actinomyces in the study area.10 actinomycetes with high antimicrobial activities were obtained from the desert salt soil of Xinjiang.
中文关键词	荒漠盐碱环境 ; 动物病原菌 ; 拮抗性放线菌 ; 分类鉴定
英文关键词	saline-alkali soil in desert animal pathogen antagonistic actinomycetes taxonomic identification
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY ; MICROBIOLOGY
WOS研究方向	Agriculture ; Microbiology
CSCD记录号	CSCD:4783291
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/230291
作者单位	1.西北农林科技大学资源环境学院, 杨凌, 陕西 712100, 中国; 2.西北农林科技大学生命科学学院, 杨凌, 陕西 712100, 中国
推荐引用方式 GB/T 7714	马玥,来航线,韦小敏,等. 新疆荒漠盐碱环境中抗动物病原菌的放线菌筛选与鉴定[J]. 西北农林科技大学,2013,41(3):133-140.
APA	马玥,来航线,韦小敏,王海利,张珊珊,&张艳群.(2013).新疆荒漠盐碱环境中抗动物病原菌的放线菌筛选与鉴定.西北农林科技大学学报. 自然科学版,41(3),133-140.
MLA	马玥,et al."新疆荒漠盐碱环境中抗动物病原菌的放线菌筛选与鉴定".西北农林科技大学学报. 自然科学版 41.3(2013):133-140.