干旱区生态环境知识资源中心(Arid): 贺兰山不同海拔典型植被带土壤微生物多样性

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	贺兰山不同海拔典型植被带土壤微生物多样性
其他题名	Soil microbial diversity under typical vegetation zones along an elevation gradient in Helan Mountains
	刘秉儒 1; 张秀珍 1; 胡天华 2; 李文金 3
来源期刊	生态学报
ISSN	1000-0933
出版年	2013
卷号	33 期号:22 页码:7211-7220
中文摘要	土壤微生物多样性在海拔梯度的分布格局研究近年来受到和植物动物一样的重视程度，但是干旱风沙区微生物多样性在海拔梯度上的多样性分布规律尚未揭示。以处于干旱风沙区的贺兰山不同海拔的6个典型植被带土壤为研究对象，利用Biolog微平板法和磷脂脂肪酸甲酯法（FAMEs）系统研究微生物多样性群落特征以及在不同植被带分布规律。结果表明：土壤微生物功能多样性随海拔增加发生变化，且微生物群落结构存在显著差异。Biolog分析显示土壤微生物群落代谢活性依次是：亚高山草甸 > 寒温性针叶林 > 针阔混交林 > 温性针叶林 > 山地旱生灌丛 > 荒漠草原，随海拔的升高土壤微生物群落物种丰富度指数（H）和均匀度指数（E）总体上均表现出增大的趋势，差异显著（P < 0.05）；FAMEs分析表明不同海拔的微生物区系发生了一定程度的变化，寒温性针叶林土壤微生物磷酸脂肪酸生物标记的数量和种类均最高，且细菌、真菌特征脂肪酸相对含量也最高；土壤微生物群落结构多样性次序是：寒温性针叶林带 > 针阔混交林带 > 温性针叶林带 > 亚高山草甸 > 山地旱生灌丛 > 荒漠草原。研究结果表明贺兰山海拔梯度的微生物多样性分布规律不同于已有的植物多样性中部膨胀研究结果，这说明在高海拔地区有更多的适合该生境的微生物存在，这对维持干旱风沙区的生态系统功能稳定性具有重要意义。
英文摘要	The study of elevational diversity gradients dates back to the foundation of biogeography. Elevational diversity patterns on plant and animal taxa have been studied exclusively over the past century, however, pattern of soil microbial diversity along an elevation gradient remain poorly understood, especially in the arid sand areas. Helan Mountain is one of few well conserved natural ecosystems in North-West of China, which have the characteristic of the significant vertical distribution of vegetation and the vegetation horizontal zonation from desert to frigid zones. Therefore, it will provide us an idea study model to assess the generality of elevational diversity patterns for soil bacterial diversity along an elevation gradient. Here, we present a comprehensive analysis of soil bacterial community composition and diversity along six elevations representing six typical vegetation types from desert steppe to subalpine meadow. The structure and function of soil microbial community were studied by using Biolog plate and phospholipid fatty acid methyl ester (FAMEs), respectively. The results showed that soil microbial functional diversity and community structure differed significantly along the elevation gradient (vegetation types). Shannon-Weiner index (H) of soil microbe and species evenness index (E) showed an increasing trend (P < 0.05). The order of microbial metabolic activity was subalpine meadow > cold temperate coniferous forest > coniferous mixed forest > temperate coniferous forest > arid dwarf-scrub > desert steppe, thus the maximal microbial metabolic activity would alter soil C and N cycling to meet available nutrients for plants in the high altitude sites, because soil N and P increased with the elevation The FAMEs analysis showed that micro flora shifted along the elevational gradient and soil bacteria dominated microbial community, however, differed significantly along the elevation gradient (vegetation types). Soil microbial diversity and number and type of phospholipid fatty acid biomarkers were the highest in the cold temperate coniferous forest. The relative contents of phospholipid fatty acid in bacteria, fungi were also the highest in the cold temperate coniferous forest. The order of microbial community diversity was cold temperate coniferous forest > coniferous mixed forest > temperate coniferous forest > subalpine meadow > mountain xerophytic shrub > desert steppe, which indicated that more litter or organic resource in forest ecosystems than grasslands would favor soil microbial diversity. These results supported that microbes do not follow the elevational diversity patterns of plants and the difference could be caused by ecological and evolutionary processes across the gradient. The finding suggests that there were more suitable microorganisms, which played more important in maintaining ecosystem stability in the arid sand areas.
中文关键词	贺兰山 ; 海拔梯度 ; 植被带 ; 土壤微生物多样性 ; 磷脂脂肪酸甲酯法
英文关键词	Biolog Helan Mountains elevation gradient vegetation zone soil microbial diversity biolog FAMEs
语种	中文
国家	中国
收录类别	CSCD
WOS类目	BIOLOGY
WOS研究方向	Life Sciences & Biomedicine - Other Topics
CSCD记录号	CSCD:5001964
来源机构	兰州大学 ; 宁夏大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/230049
作者单位	1.宁夏大学, 西北退化生态系统恢复与重建教育部重点实验室, 银川, 宁夏 750021, 中国; 2.宁夏贺兰山国家级自然保护区管理局, 银川, 宁夏 750021, 中国; 3.兰州大学, 草地农业生态系统国家重点实验室, 兰州, 甘肃 730000, 中国
推荐引用方式 GB/T 7714	刘秉儒,张秀珍,胡天华,等. 贺兰山不同海拔典型植被带土壤微生物多样性[J]. 兰州大学, 宁夏大学,2013,33(22):7211-7220.
APA	刘秉儒,张秀珍,胡天华,&李文金.(2013).贺兰山不同海拔典型植被带土壤微生物多样性.生态学报,33(22),7211-7220.
MLA	刘秉儒,et al."贺兰山不同海拔典型植被带土壤微生物多样性".生态学报 33.22(2013):7211-7220.