干旱区生态环境知识资源中心(Arid): 3个树种对不同程度土壤干旱的生理生化响应

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	3个树种对不同程度土壤干旱的生理生化响应
其他题名	Physiological and biochemical responses to different soil drought stress in three tree species
	吴芹; 张光灿; 裴斌; 方立东
来源期刊	生态学报
ISSN	1000-0933
出版年	2013
卷号	33 期号:12 页码:3648-3656
中文摘要	利用盆栽试验和采用人工浇水后持续自然耗水的方法,研究了3年生山杏(Prunus sibirica)、沙棘(Hippophae rhamnoides)、油松(Pinus tabulaeformis)对土壤干旱胁迫的生理生化响应过程。结果表明:随着干旱程度加剧,3个树种叶片相对含水量(LRWC)及叶绿素(Chl)含量持续下降,细胞膜透性逐渐增大;其中,以山杏和沙棘变化幅度较大,油松最小;3个指标发生显著变化的土壤相对含水量(RWC)临界值,油松为54.7%、山杏和沙棘分别为46.7%和48.4%。3个树种超氧化物歧化酶(SOD)与过氧化物酶(POD)两种抗氧化酶活性,随RWC降低先升高后降低;维持较高抗氧化酶活性的RWC范围,油松为32.9%-76.4%、山杏和沙棘分别为46.7%-77.9%和35.8%-78.2%;在不同土壤干旱程度下,油松的抗氧化酶活性水平高于沙棘和山杏。3个树种两种渗透调节物质脯氨酸(Pro)与可溶性糖(Ss)的含量随RWC降低而增加,油松的增加幅度大于山杏和沙棘;Pro含量的增加速度大于Ss含量。结论:3个树种可通过渗透调节、抗氧化酶活性升高等途径增强对干旱逆境的耐受性和适应性;3个树种的抗旱能力为油松>沙棘>山杏。
英文摘要	Water deficiency plays an important role in plant growth and metabolism. Response and adaptation to drought stress has become one of the hotspots of plant physiological research. Prunus sibirica、Hippophae rhamnoides and Pinus tabulaeformis are common afforestational tree species in arid and semi-arid region of North China.However, changes of the physiological and biochemical responses to different soil drought stress and their drought resistance are not clear at present.The objective of this study is to explore the quantitative relationship of the changes of the physiological and biochemical indexes and the soil moisture and to evaluate the drought-resistant ability of the three tree species. It will help us to further understand their physiological and ecological characteristics of the water and drought-resistant physiology. The results will also provide guidance for the cultivation of the three tree species in arid area. The physiological and biochemical responses of three-year-old of P. sibirica, H. rhamnoides and P. tabulaeformis to soil drought stress were investigated by natural drought method. The results showed that leaf relative water content (LRWC) and the chlorophyll (Chl) content decreased while the cell membrane permeability increased gradually in the three tree species following the relative water content (RWC) of soil reduced. The changes of the tested responses in P. sibirica and H. rhamnoides were bigger than those in P. tabulaeformis.LRWC, Chl content and the cell membrane relative permeability changed significantly when the soil RWC were lower than 54.7%, 46.7%, and 48.4% for P. tabulaeformis, P. sibirica and H. rhamnoides, respectively.Superoxide dismutase (SOD) and peroxidase (POD) which are the most important protective enzymes to eliminate reactive oxygen species (ROS) maintain higher activity to prevent ROS poisoned. The activity of SOD and POD in the three tree species increased first and then decreased following the soil water reduced.The response time and the defensive role of SOD and POD to drought stress were different. SOD played an more important role in the early drought stress and POD played a bigger role in the late drought stress when drought stress was intensified.The soil RWC required to maintain higher enzyme activity were 32.9%-76.4%, 46.7%-77.9% and 35.8%-78.2% for P. tabulaeformis, P. sibirica, and H. rhamnoides, respectively. Osmotic regulation is also an important physiological mechanism of plants adapted to drought stress. Plants can reduce the osmotic potential and maintain cell turgidity through the accumulation of the solute, such as the soluble sugar (Ss) and the proline (Pro) which can help plant to resist and adapt to drought stress. The Pro content and the Ss content increased following the soil water reduced, with the higher level in P. tabulaeformis. The Pro increased faster than Ss. So we conclude that the three tree species enhance their tolerance and adaptability to drought stress by osmotic adjustment and protective enzyme system.The comprehensive evaluation was made about the drought-resistant capability of the three tree species by using the subject function method. The order was P. tabulaeformis>H. rhamnoides>P. sibirica.
中文关键词	抗旱生理 ; 水分胁迫 ; 抗氧化酶活性 ; 渗透调节 ; 抗旱评价
英文关键词	drought-resistant physiology water stress antioxidant enzyme activity osmotic regulation drought resistance evaluation
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FORESTRY
WOS研究方向	Forestry
CSCD记录号	CSCD:4859053
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/230001
作者单位	山东农业大学林学院,国家林业局泰山森林生态站, 山东省土壤侵蚀与生态修复重点实验室, 泰安, 山东 271018, 中国
推荐引用方式 GB/T 7714	吴芹,张光灿,裴斌,等. 3个树种对不同程度土壤干旱的生理生化响应[J],2013,33(12):3648-3656.
APA	吴芹,张光灿,裴斌,&方立东.(2013).3个树种对不同程度土壤干旱的生理生化响应.生态学报,33(12),3648-3656.
MLA	吴芹,et al."3个树种对不同程度土壤干旱的生理生化响应".生态学报 33.12(2013):3648-3656.