干旱区生态环境知识资源中心(Arid): HPLC-MS/MS法测定水产品中硫酸粘素、杆菌肽及维吉尼霉素M

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	HPLC-MS/MS法测定水产品中硫酸粘素、杆菌肽及维吉尼霉素M_1的残留量
其他题名	Determination of colistin sulfate, bacitracin and virginiamycin M_1 residues in aquatic products by HPLC-MS/MS
	罗方方 1; 钱卓真 2; 林荣晓 3; 吴成业 2
来源期刊	南方水产科学
ISSN	2095-0780
出版年	2013
卷号	9 期号:4 页码:63-69
中文摘要	建立了水产品中硫酸粘菌素(CS)、杆菌肽(BTC)及维吉尼霉素M1(VGMM1) 3种多肽类抗生素残留量检测的HPLC-MS/MS法。样品经水溶液[V(甲醇) : V(0.1%甲酸水溶液)=2:5]提取,4%三氯乙酸乙腈除蛋白,乙腈饱和正己烷除脂,过OASIS HLB(60 mg)小柱净化后,利用HPLC-MS/MS法,以选择反应监测模式检测,外标法进行定量分析。CS和BTC在0.01~10.00 mg?L~(-1)质量浓度范围内线性良好,VGMM_1在0.002~2.000 mg?L~(-1)质量浓度范围内线性良好,R~2均大于0.995;3种多肽类抗生素的检出限分别为CS 10 mug?kg~(-1)、BTC 10 mug?kg~(-1)、VGMM1 2 mug?kg~(-1),定量限分别为20 mug?kg~(-1)、20 mug?kg~(-1)和4 mug?kg~(-1);选择3个不同浓度水平做加标回收,平均回收率在72.3%~103.9%,相对标准偏差为1.10% ~ 10.92%。该方法具有操作简便,准确性好,灵敏度高,重现性好等优点,可为检测水产品中这3种药物的残留提供相关技术支持。
英文摘要	A high performance liquid chromatography tandem mass spectrometric (HPLC-MS/MS) method was established to simultaneously detect colistin sulfuric (CS), bacitracin (BTC) and virginiamycin M_1 (VGMM_1) in aquatic products. We extracted the samples with methanol- 0.1% formicacid(V:V=2:5), removed their protein with 4% TCA acetonitril, removed fat with acetonitrile saturated n-hexane, and then cleaned them up with OASiS HLB solid phase extraction (SPE) column. Next, we detected the analytes by HPLC-MS/MS under the selected reaction monitoring mode and quantified them by external standard method. Results show that CS and BTC have good linearity between the peak areas in concentrations ranged 0.01~10.00 mg?L~(-1), VGMM1 as well in concentrations ranged 0.002~2.000 mg?L~(-1), and the correlation coefficients (R~2) are all more than 0.995. The detection limits of CS, BTC and VGMM1 are 10 mug?kg~(-1), 10 mug?kg~(-1)and 2 mug?kg~(-1) respectively, and the quantification limits are 20 mug?kg~(-1), 20 mug?kg~(-1) and 4 mug?kg~(-1)respectively. Three samples in three different concentration levels are chosen for recovery, getting a 72.3%~103.9% average recovery rate, and a relative standard deviations varying from 1.10%~10.92%. In conclusion, method proposed has advantages of simplicity, sensitivity and repeatability, which provide technical support for the detection of these 3 kinds of drug residues in aquatic products.
中文关键词	硫酸粘菌素 ; 杆菌肽 ; 维吉尼霉素M1 ; 水产品 ; 液相色谱串联质谱法
英文关键词	colistin sulfate bacitracin virginiamycin M1 aquatic products liquid chromatography tandem mass spectrometry
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FOOD SCIENCE TECHNOLOGY
WOS研究方向	Food Science & Technology
CSCD记录号	CSCD:4922159
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/229858
作者单位	1.集美大学生物工程学院, 厦门, 福建 361021, 中国; 2.福建省水产研究所, 厦门, 福建 361012, 中国; 3.福建农林大学, 福州, 福建 350002, 中国
推荐引用方式 GB/T 7714	罗方方,钱卓真,林荣晓,等. HPLC-MS/MS法测定水产品中硫酸粘素、杆菌肽及维吉尼霉素M_1的残留量[J],2013,9(4):63-69.
APA	罗方方,钱卓真,林荣晓,&吴成业.(2013).HPLC-MS/MS法测定水产品中硫酸粘素、杆菌肽及维吉尼霉素M_1的残留量.南方水产科学,9(4),63-69.
MLA	罗方方,et al."HPLC-MS/MS法测定水产品中硫酸粘素、杆菌肽及维吉尼霉素M_1的残留量".南方水产科学 9.4(2013):63-69.