干旱区生态环境知识资源中心(Arid): 液相色谱-串联质谱法测定化妆品中22种激素

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	液相色谱-串联质谱法测定化妆品中22种激素
其他题名	LC-MS/MS Determination of 22 Hormones in Cosmetics
	陈溪 1; 张晓林 1; 刘莹 1; 崔晗 1; 黄大亮 1; 林维宣 2
来源期刊	理化检验. B, 化学分册
ISSN	1001-4020
出版年	2013
卷号	49 期号:4 页码:468-472,476
中文摘要	提出了采用液相色谱-串联质谱法测定化妆品中22种激素残留量的方法。样品经乙腈超声提取,分别采用OasisHLB固相萃取柱(方法一)和凝胶渗透色谱(方法二)两种方法净化。经净化的试液用ZORBAXEclipseXDB-C_(18)色谱柱(100mm*2.1mm,1.8mum)分离,15种激素用不同比例配成的含0.1%(体积分数)甲酸的水溶液和乙腈组成的流动相梯度洗脱。质谱测定中采用正离子电离方式多反应监测模式;另7种激素用不同比例配成的水和乙腈组成的流动相梯度洗脱,质谱测定中采用负离子电离方式多反应监测模式。方法一的检出限为0.01?0.40mg?kg~(-1);回收率在61.5%?111%之间;方法二的检出限在0.1?4.0mg?kg~(-1)之间,回收率在60.8%?113%之间。
英文摘要	LC-MS/MS was applied to the determination of residual amounts of 22 hormones in cosmetics. The sample was ultrasonically extracted with acetonitrile, and purified by Oasis HLB solid-phase extraction column (method 1) and gel permeation chromatography (method 2) separately. The hormones in the purified solution were separated on ZORBAX Eclipse XDB-C_(18) (100 mm*2.1 mm, 1.8mum) column and eluted by gradient elution. Mixtures of acetonitrile and 0.1% (cp) formic acid solution with different mixing ratio by volume were used as eluants in the elution, and ESI~+ mode was used in MS determination for 15 of the 22 hormones, and mixtures of acetonitrile and H_2O with different mixing ratio by volume were used as eluants in the elution and ESI~- mode was used in MS determination for the remainder 7 hormones. Multi-reaction monitoring mode was adopted in both the above cases. Values of detection limit (3S/N) found for method 1 were in the range of 0.01 0. 40 mg ? kg~(-1), while for method 2.were in the range of 0.14.0 mg ? kg~(-1). Values of recovery found for method 1 were in the range of 61.5%?111%, while for method 2 were in the range of 60.8%113%.
中文关键词	液相色谱-串联质谱法 ; 凝胶渗透色谱 ; 化妆品 ; 激素
英文关键词	LC-MS/MS GPC Cosmetic Hormones
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4850780
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/229838
作者单位	1.大连出入境检验检疫局庄河办事处, 庄河, 116400; 2.辽宁出入境检验检疫局, 大连, 辽宁 116000, 中国
推荐引用方式 GB/T 7714	陈溪,张晓林,刘莹,等. 液相色谱-串联质谱法测定化妆品中22种激素[J],2013,49(4):468-472,476.
APA	陈溪,张晓林,刘莹,崔晗,黄大亮,&林维宣.(2013).液相色谱-串联质谱法测定化妆品中22种激素.理化检验. B, 化学分册,49(4),468-472,476.
MLA	陈溪,et al."液相色谱-串联质谱法测定化妆品中22种激素".理化检验. B, 化学分册 49.4(2013):468-472,476.