干旱区生态环境知识资源中心(Arid): 内蒙古亚洲小车蝗种群遗传多样性的微卫星分析

Arid

	内蒙古亚洲小车蝗种群遗传多样性的微卫星分析
其他题名	Microsatellite marker analysis of the genetic diversity of Oedaleus asiaticus (Orthoptera: Acrididae) populations in Inner Mongolia, northern China
	韩海斌; 周晓榕; 庞保平; 张敏哲; 李海平
来源期刊	昆虫学报
ISSN	0454-6296
出版年	2013
卷号	56 期号:1 页码:79-87
中文摘要	亚洲小车蝗Oedaleus asiaticus Bei-Bienko是我国北方草原和农牧交错区主要的害虫之一。为了从分子水平评价内蒙古地区亚洲小车蝗种群的遗传多样性和种群间遗传分化,本文采用8对微卫星引物对内蒙古15个地点的亚洲小车蝗种群进行遗传多样性分析。结果表明:各位点有效等位基因数为3.4517~13.2881,多态信息含量值为0.5601~0.8563,Shannon氏多样性指数在0.7018~4.1789之间。15个种群的平均期望杂合度为0.6836,Nei氏期望杂合度为0.5303~0.6513,群体遗传距离为0.1092~0.4235,群体分化率Fst平均值为0.1612,基因流Nm平均值为1.6164。8个微卫星位点均具有较高的多态性,各种群间的遗传分化水平较大,基因交流程度中等,个体间的遗传变异大于种群间的遗传变异。15个不同地点的亚洲小车蝗种群根据遗传距离共聚为6支。种群间遗传分化与地理距离呈正相关关系。高山和沙漠对不同地区亚洲小车蝗种群的迁移具有阻碍作用,可能是形成遗传分化的主要原因。研究结果从分子水平探索不同地区亚洲小车蝗种群间的内在联系,为制定亚洲小车蝗的综合治理策略提供了分子生物学的基础资料。
英文摘要	Oedaleus asiaticus Bei-Bienko is one of the major pest insects in the grasslands and agropastoral ecotone in North China. By using 8 microsatellite primer pairs,the genetic diversities of 15 geographic populations of O. asiaticus in Inner Mongolia,northern China were analyzed in order to evaluate the genetic variability within populations and genetic differentiation among populations of O. asiaticus at the molecular level. The results showed that the effective number of alleles was 3. 4517 - 13. 2881,the polymorphic information content was 0. 5601 - 0. 8563 and the Shannons diversity index was 0. 7018 - 4. 1789. The mean expected heterozygosity of the 15 populations was 0. 6836,with the Neis expected heterozygosity of 0. 5303 - 0. 6513 and genetic distance of 0. 1092 - 0. 4235. The mean fixation index (Fst) and the mean gene flow (Nm) were 0. 1612 and 1. 6164,respectively. The 8 microsatellite loci selected are of high polymorphism,this grasshopper keeps a high genetic differentiation and moderate gene flow among populations,and the genetic variability among individuals within a population is higher than that among populations. O. asiaticus populations from 15 areas were classified into 6 groups according to the genetic distance. Genetic differentiation between populations shows a positive correlation with geographical distance. High mountains and deserts always block the population migration between areas,which is possibly the main cause of genetic differentiation. The study investigates the intrinsic connection among populations from different areas at the molecular level and provides some basic information on molecular biology for developing integrated management strategies against O. asiaticus.
中文关键词	亚洲小车蝗 ; 地理种群 ; 遗传多样性 ; 微卫星标记 ; 遗传分化 ; 聚类分析
英文关键词	Oedaleus asiaticus geographic population genetic diversity microsatellite marker genetic differentiation cluster analysis
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENTOMOLOGY
WOS研究方向	Entomology
CSCD记录号	CSCD:4769050
来源机构	内蒙古农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/229821
作者单位	内蒙古农业大学农学院, 呼和浩特, 内蒙古 010019, 中国
推荐引用方式 GB/T 7714	韩海斌,周晓榕,庞保平,等. 内蒙古亚洲小车蝗种群遗传多样性的微卫星分析[J]. 内蒙古农业大学,2013,56(1):79-87.
APA	韩海斌,周晓榕,庞保平,张敏哲,&李海平.(2013).内蒙古亚洲小车蝗种群遗传多样性的微卫星分析.昆虫学报,56(1),79-87.
MLA	韩海斌,et al."内蒙古亚洲小车蝗种群遗传多样性的微卫星分析".昆虫学报 56.1(2013):79-87.