干旱区生态环境知识资源中心(Arid): SPE-RRLC-MS/MS法测定制药废水中青霉素G

Arid

	SPE-RRLC-MS/MS法测定制药废水中青霉素G
其他题名	DETERMINATION OF PENICILLIN G IN PHARMACEUTICAL WASTEWATER BY SPE-RRLC-TANDEM MS
	李再兴 1; 张非非 1; 左剑恶 2; 王勇军 3; 陈平 3; 周崇晖 3; 余忻 2
来源期刊	环境工程
ISSN	1000-8942
出版年	2013
卷号	31 期号:3 页码:135-138
中文摘要	建立了利用固相萃取-高分离度快速液相色谱-串联质谱(SPE-RRLC-MS/MS)测定制药废水中青霉素G残留的方法。水样以30%硫酸锌+20%亚铁氰化钾作为沉淀剂沉淀蛋白质后,上清液采用Oasis HLB固相萃取柱富集和净化,以0.01 mol/L乙酸铵溶液(加0.1%甲酸)+乙腈作为流动相进行等度洗脱,经Agilent Plus C_(18)柱分离后,在串联质谱ESI(+)源下进行MRM检测。该方法的青霉素G检出限(S/N=3)为0.02mug/L,目标物在0.0020~1.0 mg/L范围内线性关系良好,线性相关系数为0.9997。在0.20,1.0,2.0mug/L添加水平平均回收率为74.6%~101.4%,相对标准偏差为4.49%~8.17%。该方法灵敏度高、重现性好、定性定量准确,可满足制药废水中青霉素G残留检测的要求。
英文摘要	A method based on solid phase extraction-rapid resolution liquid chromatography-tandem mass spectrometry ( SPERRLC-MS /MS) was developed to determine penicillin G in pharmaceutical wastewater. Water samples were firstly purified through protein precipitation processes with 30% zinc sulfate and 20% potassium ferrocyanide,and then the penicillin G was enriched and purified with Oasis HLB SPE,separated through Agilent Plus C_(18) RRLC using 0. 01 mol /L ammonium acetate solution( 0. 1% formic acid) and acetonitrile as mobile phase,and detected by mass spectrometry in positive ion mode with multiple reaction monitoring (MRM) using an electrospray ionization interface. The detection limit( S /N = 3) was 0. 02 mug / L. The linear range was 0. 0020mg /L to 1. 0 mg /L,with a correlation coefficient of 0. 9997. The recoveries at the spiked concentration of 0. 20 mug /L,1. 0 mug /L and 2. 0 mug /L ranged from 74. 6% to 101. 4%,with a relative standard deviation from 4. 49% to 8. 17%. The procedure provides a rapid,reliable and sensitive method for determination of penicillin G in pharmaceutical wastewater
中文关键词	青霉素G ; 高分离度快速液相色谱- 串联质谱 ; 固相萃取 ; 制药废水
英文关键词	penicillin G rapid resolution liquid chromatography-tandem mass spectrometry ( RRLC-MS /MS ) solid phase extraction ( SPE) pharmaceutical wastewater
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENVIRONMENTAL SCIENCES ; CHEMISTRY ANALYTICAL
WOS研究方向	Environmental Sciences & Ecology ; Chemistry
CSCD记录号	CSCD:4859670
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/229780
作者单位	1.河北科技大学环境科学与工程学院, 石家庄, 河北 050018, 中国; 2.清华大学环境学院, 北京 100084, 中国; 3.华北制药集团有限责任公司环境保护研究所, 石家庄, 河北 050015, 中国
推荐引用方式 GB/T 7714	李再兴,张非非,左剑恶,等. SPE-RRLC-MS/MS法测定制药废水中青霉素G[J],2013,31(3):135-138.
APA	李再兴.,张非非.,左剑恶.,王勇军.,陈平.,...&余忻.(2013).SPE-RRLC-MS/MS法测定制药废水中青霉素G.环境工程,31(3),135-138.
MLA	李再兴,et al."SPE-RRLC-MS/MS法测定制药废水中青霉素G".环境工程 31.3(2013):135-138.