干旱区生态环境知识资源中心(Arid): 超高效液相色谱-串联质谱法测定玉米中硝磺草酮及其代谢物残留量

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	超高效液相色谱-串联质谱法测定玉米中硝磺草酮及其代谢物残留量
其他题名	Determination of Mesotrione and Its Metabolite Residues in Maize by Ultra Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry
	邓立刚 1; 李增梅 2; 赵善仓 2; 陈业兵 2; 郭长英 2; 路福绥 3
来源期刊	分析化学
ISSN	0253-3820
出版年	2013
卷号	41 期号:8 页码:1269-1273
中文摘要	建立了超高效液相色谱-串联质谱法测定玉米中硝磺草酮及其代谢物(MNBA)的残留分析方法。样品用乙腈-水溶液提取,取适量提取液用甲酸酸化至pH 2,过HLB固相萃取柱进行净化,用甲醇-乙醚(70∶30,V/V)洗脱,洗脱液用氮气吹至近干,用1 mmol/L醋酸铵-乙腈(25∶75,V/V)定容后进行质谱分析,利用基质校正曲线对其准确定量。硝磺草酮和MNBA在1~200mug/L浓度范围内呈线性关系,相关系数均大于0.99,在添加浓度5~50mug/kg范围内,硝磺草酮及其代谢物MNBA的平均回收率为83.8%~101.1%,相对标准偏差在5.3%~12.8%之间,硝磺草酮和代谢物MNBA定量限分别为1.0和2.0mug/kg。
英文摘要	A novel method was developed for the simultaneous determination of mesotrione and its metabolite (4-methylsulfonyl-2-nitrobenzoic acid, MNBA) in maize by ultra performance liquid chromatography coupled with tandem mass spectrometry(UPLC-MS/ MS). The samples were extracted with acetonitrile-water (50:50, V / V) solutions and an aliquot of the extract was acidified to pH 2 with formic acid and then cleaned-up by Oasis HLB cartridge. The residues were eluted from cartridge with methanol-diethyl ether (70:30, V / V). The elutions were evaporated to appropriately dry under a stream of nitrogen, dissolved in 2 mL acetonitrile1 mmol/ L ammonium acetate (75:25, V / V). The residues were analyzed by mass spectrometry with negative ion mode using multiple-reaction monitoring (MRM) and quantification was achieved by matrix calibration. The method had a good linear correlation (correlation coefficient was above 0. 99) in the range of 1-200 mug/ L. The average recoveries of mesotrione and MNBA were 83. 8% -101. 1% at spiked level between 5 mug/ kg and 50 mug/ kg with the relative standard deviation (RSD) between 5. 3% and 12. 8%. The limits of quantification for mesotrione and MNBA were 1. 0 and 2. 0 mug/ kg, respectively.
中文关键词	硝磺草酮 ; 液相色谱-串联质谱 ; 玉米 ; 代谢物
英文关键词	Mesotrione Liquid chromatography-tandem mass spectrometry Maize Metabolite
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:4912464
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/229457
作者单位	1.山东农业大学植物保护学院, 山东省食品质量与安全检测技术重点实验室, 泰安, 山东 271018, 中国; 2.山东省农业科学院农业质量标准与检测技术研究所, 山东省食品质量与安全检测技术重点实验室, 济南, 山东 250100, 中国; 3.山东农业大学植物保护学院, 泰安, 山东 271018, 中国
推荐引用方式 GB/T 7714	邓立刚,李增梅,赵善仓,等. 超高效液相色谱-串联质谱法测定玉米中硝磺草酮及其代谢物残留量[J],2013,41(8):1269-1273.
APA	邓立刚,李增梅,赵善仓,陈业兵,郭长英,&路福绥.(2013).超高效液相色谱-串联质谱法测定玉米中硝磺草酮及其代谢物残留量.分析化学,41(8),1269-1273.
MLA	邓立刚,et al."超高效液相色谱-串联质谱法测定玉米中硝磺草酮及其代谢物残留量".分析化学 41.8(2013):1269-1273.