干旱区生态环境知识资源中心(Arid): UPLC-MS/MS法检测肉类组织中的11种全氟化合物

Arid

	UPLC-MS/MS法检测肉类组织中的11种全氟化合物
其他题名	Determination of Eleven Perfluorinated Compounds in Meat by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
	刘莉治 1; 郭新东 2; 方军 3; 冼燕萍 2; 黄聪 1; 彭荣飞 1; 罗晓燕 1
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2013
卷号	32 期号:7 页码:862-866
中文摘要	建立了肉类组织中11种常见全氟化合物(全氟丁烷磺酸、全氟己烷磺酸、全氟庚酸、全氟辛酸、全氟辛烷磺酸、全氟壬酸、全氟癸酸、全氟十一酸、全氟癸烷磺酸、全氟十二酸、全氟十四酸)的Waters Oasis HLB固相萃取净化/超高效液相色谱-串联质谱(UPLC-MS/MS)分析方法。肉类组织中加入内标,经乙腈均质、提取后,用玻璃纤维真空抽滤,再用乙腈饱和的正己烷除脂净化,经Waters Oasis HLB固相萃取小柱富集净化,氮吹浓缩,超高效液相色谱(UPLC)分离,以2 mmol/L醋酸铵-2 mmol/L醋酸铵甲醇溶液为流动相,使用串联四极杆电喷雾离子源负离子扫描,多反应监测(MRM)模式检测,内标法定量。11种全氟化合物在0.50 ~20.0 mug/L范围内线性关系良好,方法的检出限为0.014~0.060 mug/kg,定量下限为0.047 ~0.199 mug/kg,回收率为70.9%~107.1%,相对标准偏差(RSD,n=6)为0.4% ~11.4%。该方法具有净化效果好、定量准确、灵敏度高的特点,适用于肉类组织中11种常见全氟化合物的快速确认和准确定量。
英文摘要	A method was developed for the determination of 11 perfluorinated compounds (PFCs) (e.g.perfluorobutanesulfonate,perfluorohexane-sulfonate,perfluoroheptanoic-acid,perfluorooctanoate,perfluorooctane-sulfonate,perfluorononanoic-acid,perluorodecanoic-acid,perfluoroundecanoic-acid,perfluorodecanesulfonate,perfluorododecanoic-acid and perfluorotetradecanoic acid)in meat by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) with solid phase extraction column.After added with the isotopic labeled sodium perfluoro-1-[1,2,3,4,-~(13)C_4]octanesulfonat (MPFOA) and perfluoro-n-[1,2,3,4,-~(13)C_4]octanoic acid (MPFOS),the samples were extracted and homogenized with acetonitrile,and then suction filted through glass fiber and defatted with n-hexane by liquid-liquid extraction method.The extract was purified with Waters Oasis HLB solid phase extraction column,and concentrated with N_2.The perfluorinated compounds were separated with UPLC using a mixture of 2 mmol/L ammonium acetate-2 mmol/L ammonium acetate methanol solution as mobile phase,and analysed by UPLC-MS/MS ESI(-) under MRM mode with the internal standard method.The calibration curves were linear in the range of 0.50-20.0 mug/L for 11 PFCs.The limits of detection for the method (LODs) were between 0.014 mug/kg and 0.060 mug/kg,and the limits of quantitation were between 0.047 mug/kg and 0.199mug/kg.The mean recoveries were in the range of 70.9%-107.1%,with relative standard deviations (RSDs,n=6) of 0.4% -11.4%.This method was suitable for the identification and quantification of 11 perfluorinated compounds in meat due to its simplicity,accuracy and high sensitivity.
中文关键词	全氟化合物 ; 肉 ; 超高效液相色谱-串联质谱法 ; 固相萃取
英文关键词	perfluorinated compounds meat ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS) solid phase extraction
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4896332
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/229451
作者单位	1.广州市疾病预防控制中心, 广州, 广东 510440, 中国; 2.广州市质量监督检测研究院, 广州, 广东 510110, 中国; 3.中国广州分析测试中心, 广州, 广东 510070, 中国
推荐引用方式 GB/T 7714	刘莉治,郭新东,方军,等. UPLC-MS/MS法检测肉类组织中的11种全氟化合物[J],2013,32(7):862-866.
APA	刘莉治.,郭新东.,方军.,冼燕萍.,黄聪.,...&罗晓燕.(2013).UPLC-MS/MS法检测肉类组织中的11种全氟化合物.分析测试学报,32(7),862-866.
MLA	刘莉治,et al."UPLC-MS/MS法检测肉类组织中的11种全氟化合物".分析测试学报 32.7(2013):862-866.