干旱区生态环境知识资源中心(Arid): 高效液相色谱-紫外法同时检测小麦中DON、15ACDON和3ACDON

Arid

	高效液相色谱-紫外法同时检测小麦中DON、15ACDON和3ACDON
其他题名	Establishment of a HPLC-UV Method for Simultaneous Determination of DON,15ACDON,and 3ACDON in Wheat
	杨丹; 耿志明; 马鸿翔; 姚金保; 张旭; 张平平; 张鹏
来源期刊	作物学报
ISSN	0496-3490
出版年	2012
卷号	38 期号:1 页码:186-189
中文摘要	建立了测定小麦中B型单端孢酶烯族毒素脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)、3-乙酰脱氧雪腐镰刀菌烯醇(3-acetyldeoxynivalenol,3-ACDON)和15-乙酰脱氧雪腐镰刀菌烯醇(15-acetyldeoxynivalenol,15-ACDON)的高效液相色谱-紫外(HPLC-UV)检测方法。用水提取小麦样品,提取液经无水乙醇等体积沉淀,再结合OasisHLB固相萃取小柱可取得较好的净化效果。采用乙腈-0.005%磷酸水溶液二元梯度洗脱程序在高效液相色谱-紫外检测器上完成DON、15ACDON和3ACDON的定性定量分析。结果表明,在0.5~15.0mgL~(-1)线性范围内,DON、15ACDON和3ACDON的平均加标回收率分别为89.8%、93.4%和92.9%,相对标准偏差分别为2.2%、2.0%和2.5%(n=3);检测限分别为12.2、10.5和16.7mugkg-1。该方法准确、重现性好,样品净化方法使杂峰干扰少,大幅减少有机溶剂的使用,成本低,适用于小麦中B型单端孢酶烯族毒素的大批量检测。
英文摘要	A HPLC-UV method was established to determine deoxynivalenol(DON),3-acetyldeoxynivalenol(3-ACDON) and 15-acetyldeoxynivalenol(15-ACDON) in wheat.Wheat sample was extracted by water,and the extracting solution was precipitated with an equal volume of ethanol followed by solid phase extraction with Oasis HLB cartridge.Qualitative and quantitative analyses of DON,15ACDON and 3ACDON were done by HPLC-UVD using gradient elution program with acetonitrile and 0.005% phosphoric acid solution.Results showed that at the linear range of 0.5-15.0 mg L 1,the recoveries were 89.8%,93.4%,and 92.9%,respectively.The relative standard deviations were 2.2%,2.0%,and 2.5%,respectively,and the detection limit levels were 12.2,10.5,and 16.7 mug kg 1,respectively.The method was accurate and reproducible.Sample purification lessened interference peaks,and due to the use of organic solvents was greatly reduced,the cost was decreased.Therefore,the method was suitable for the large batch determination of type B trichothecenes in wheat.
中文关键词	小麦 ; B型单端孢酶烯族毒素 ; 高效液相色谱-紫外检测法
英文关键词	Wheat B trichothecenes HPLC-UV
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRONOMY
WOS研究方向	Agriculture
CSCD记录号	CSCD:4435134
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/229201
作者单位	江苏省农业科学院, 江苏省农业生物学重点实验室, 南京, 江苏 210014, 中国
推荐引用方式 GB/T 7714	杨丹,耿志明,马鸿翔,等. 高效液相色谱-紫外法同时检测小麦中DON、15ACDON和3ACDON[J],2012,38(1):186-189.
APA	杨丹.,耿志明.,马鸿翔.,姚金保.,张旭.,...&张鹏.(2012).高效液相色谱-紫外法同时检测小麦中DON、15ACDON和3ACDON.作物学报,38(1),186-189.
MLA	杨丹,et al."高效液相色谱-紫外法同时检测小麦中DON、15ACDON和3ACDON".作物学报 38.1(2012):186-189.