干旱区生态环境知识资源中心(Arid): 古尔班通古特沙漠南缘蜱携带无形体的调查

Arid

	古尔班通古特沙漠南缘蜱携带无形体的调查
其他题名	Survey on tick-borne anaplasmataceae in the south edge of Gurbantunggut desert
	孟庆玲 1; 乔军 1; 盛金良 1; 王俊伟 1; 王为升 1; 姚娜 1; 陈创夫 1; 张丽娟 2
来源期刊	中国兽医学报
ISSN	1005-4545
出版年	2012
卷号	32 期号:8 页码:1158-1163
中文摘要	为了解古尔班通古特沙漠南缘蜱携带无形体的状况,在该地域3个不同生境区域11个地点采集家畜寄生蜱标本,进行分类鉴定;运用套式PCR筛选无形体阳性蜱样本,并用半套式PCR扩增无形体科16SrRNA基因5′端高变区,进行克隆测序;将所获得的DNA序列与GenBank收录的序列作比对,并用Mega 5.0软件构建系统发育树,鉴定蜱携带无形体的种类。结果共采集708只蜱,鉴定为4个属8个种。在分类后的236份蜱样本中,检测出无形体阳性25份,阳性率为10.59%。序列比较发现,在亚洲璃眼蜱、血红扇头蜱、残缘璃眼蜱、长角血蜱中分别存在查菲埃立克体(Ehrlichia chaffeensis)、犬埃立克体(Ehrlichia canis)、边缘无形体(Anaplasma marginale)和绵羊无形体(Anaplasma ovis)的16SrRNA基因片段。研究结果证实在古尔班通古特沙漠南缘家畜寄生蜱中携带无形体,提示该地域可能是无形体病的自然疫源地。
英文摘要	To understand the tick-borne anaplasmataceae in the south edge of Gurbantunggut desert,ticks specimens from 11 sites in three different habitats areas were collected and identified.Nested PCR was carried to detect positive ticks carring anaplasmataceae,and then 5′end variable region of 16S rRNA gene of anaplasmataceae was amplified by semi-nested PCR for cloning and sequencing.The obtained DNA sequences were compared with the GenBank sequence and phylogenetic tree was constructed with the Mega 5.0 software.The 708 ticks collected from 11 sites were identified as 4 genera and 8 species.25 were detected positive in the 236 tick samples,with a positive rate of 10.59%.By sequence analyses,Ehrlichia chaf feensis,Ehrlichia canis,Anaplasma marginale and Anaplasma ovis were positive in Hyalomma asiaticum,Rhipicephalus sanguineeus,Hyalomma detritum and Haemaphysalis longicornis,respectively.The results showed that there exists anaplasmataceae in ticks parasited in livestock from the south edge of Gurbantunggut desert,suggesting that the region may be one of natural foci of anaplasmosis.
中文关键词	古尔班通古特沙漠 ; 蜱传无形体 ; 自然疫源地
英文关键词	Gurbantunggut desert tick-borne anaplasmataceae natural foci
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY ; MEDICINE GENERAL INTERNAL
WOS研究方向	Agriculture ; General & Internal Medicine
CSCD记录号	CSCD:4609640
来源机构	石河子大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/229107
作者单位	1.石河子大学动物科技学院, 石河子, 新疆 832003, 中国; 2.中国疾病预防控制中心传染病预防控制所, 北京 102206, 中国
推荐引用方式 GB/T 7714	孟庆玲,乔军,盛金良,等. 古尔班通古特沙漠南缘蜱携带无形体的调查[J]. 石河子大学,2012,32(8):1158-1163.
APA	孟庆玲.,乔军.,盛金良.,王俊伟.,王为升.,...&张丽娟.(2012).古尔班通古特沙漠南缘蜱携带无形体的调查.中国兽医学报,32(8),1158-1163.
MLA	孟庆玲,et al."古尔班通古特沙漠南缘蜱携带无形体的调查".中国兽医学报 32.8(2012):1158-1163.