干旱区生态环境知识资源中心(Arid): 气相色谱-质谱联用快速筛查尿样中麻醉剂类药物

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	气相色谱-质谱联用快速筛查尿样中麻醉剂类药物
其他题名	Fast Screening Narcotic Agents in Human Urine by GC/MS
	杨志勇; 王占良; 张建丽; 张亦农
来源期刊	质谱学报
ISSN	1004-2997
出版年	2012
卷号	33 期号:3 页码:161-167
中文摘要	为了快速检测尿样中的麻醉剂类药物(可待因,双氢可待因,四氢大麻酚酸,苯甲酰爱康宁,6-乙酰吗啡,吗啡,羟考酮),建立了气相色谱-质谱联用检测方法。尿样经葡萄糖醛酸甙酶酶解后,采取HLB Oasis反相固相萃取柱纯化,氮气吹干后衍生化,采用HP-5MS柱(25m0.2mm0.33mum)色谱分离,程序升温,质谱检测,SIM方式采集。该方法的检测限为5mg/L,在添加高、中、低3种浓度的相对回收率分布在75%~123%,满足常规检测要求,样品前处理简单、快速、结果可靠。
英文摘要	Seven narcotic agents(Codeine,Dihydrocodeine,Carboxy-THC,Benzoylecgonine,6-acetylmorphine,Morphine,and Oxycodone) were simultaneously screened by gas chromatography-mass spectrometry coupling with electron impact ionization source.The urine sample was enzymatically hydrolysised and then purified by HLB Oasis solid prepared extraction column,the analytes after derivatization were completely separated by HP-5MS column(25 m0.2 mm0.33 mum thickness) in linear temperature program,methyltestosterone as internal standard.The relative recovery of analytes spiked in blank urine at high,middle and low level concentration ranged from 75% to 123%.The limit of detection for target analytes was 5 mg/L.The method is simple,rapid and reliable.It is suitable for routine analysis in human urine.
中文关键词	气相色谱-质谱 ; 麻醉剂 ; 快速筛查 ; 尿样
英文关键词	gas chromatography-mass spectrometry(GC/MS) narcotic agents fast screening urine sample
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4569971
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/228888
作者单位	国家体育总局中国反兴奋剂中心, 北京 100029, 中国
推荐引用方式 GB/T 7714	杨志勇,王占良,张建丽,等. 气相色谱-质谱联用快速筛查尿样中麻醉剂类药物[J],2012,33(3):161-167.
APA	杨志勇,王占良,张建丽,&张亦农.(2012).气相色谱-质谱联用快速筛查尿样中麻醉剂类药物.质谱学报,33(3),161-167.
MLA	杨志勇,et al."气相色谱-质谱联用快速筛查尿样中麻醉剂类药物".质谱学报 33.3(2012):161-167.