干旱区生态环境知识资源中心(Arid): HPLC-ESI-MS/MS检测河豚鱼中5种抗生素残留的方法

Arid

	HPLC-ESI-MS/MS检测河豚鱼中5种抗生素残留的方法
其他题名	Determination of 5 Macrolide Antibiotics in Pufferfish Meat by HPLC-ESI-MS/MS
	田丰 1; 王飞 2; 吕鹏 1; 闫树峰 1; 廉力 1
来源期刊	食品科学
ISSN	1002-6630
出版年	2012
卷号	33 期号:18 页码:206-210
中文摘要	建立一种同时测定河豚鱼中林可霉素、竹桃霉素、红霉素、替米考星和泰乐菌素5种大环内酯类抗生素残留的方法。试样中残留的抗生素用Tris缓冲溶液提取,经Oasis HLB固相萃取柱净化后,用液相色谱-质谱/质谱仪检测和确证,内标法定量。结果表明:5种大环内酯类抗生素在0.002~0.050mug/mL范围内均呈线性关系,相关系数r为0.9977~1.0000;在0.002~0.010mg/kg范围内,样品平均加标回收率在78.9%~109.0%之间,重复性相对标准偏差在1.3%~5.3%之间,再现性相对标准偏差在5.2%~14.5%之间,5种大环内酯类抗生素定量限均为2.0mug/kg。
英文摘要	A method has been developed for the simultaneous determination of five marcolide antibiotic residues including lincomycin,oleandomycin,erythromycin,tilmicosin and tylosin in pufferfish meat.Five antibiotic residues in samples were extracted into Tris buffer.The extract was cleaned up on an Oasis HLB column.The antibiotic residues were determined and confirmed by LC-MS/MS and quantified by an internal standard method.The calibration curves of 5 antibiotics exhibited good linearity in the concentration range of 0.002-0.050mug/mL with a correlation coefficient range of 0.9977-1.0000.The average recovery rates of the marcolide antibiotics from pufferfish samples at spike levels of 0.002-0.010 mg/kg was 78.9%-109.0% with a relative standard deviation of 1.3%-5.3% for repeatability and with a relative standard deviation of 5.2%-14.5% for reproducibility.The detection limit was 2.0mug/kg for these 5 antibiotics.
中文关键词	液相色谱-质谱/质谱 ; 固相萃取 ; 大环内酯抗生素 ; 河豚鱼
英文关键词	high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) solid phase extraction(SPE) macrolide antibiotics pufferfish
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FOOD SCIENCE TECHNOLOGY
WOS研究方向	Food Science & Technology
CSCD记录号	CSCD:4646858
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/228537
作者单位	1.黑河出入境检验检疫局, 黑河, 黑龙江 164300, 中国; 2.秦皇岛出入境检验检疫局, 秦皇岛, 河北 066004, 中国
推荐引用方式 GB/T 7714	田丰,王飞,吕鹏,等. HPLC-ESI-MS/MS检测河豚鱼中5种抗生素残留的方法[J],2012,33(18):206-210.
APA	田丰,王飞,吕鹏,闫树峰,&廉力.(2012).HPLC-ESI-MS/MS检测河豚鱼中5种抗生素残留的方法.食品科学,33(18),206-210.
MLA	田丰,et al."HPLC-ESI-MS/MS检测河豚鱼中5种抗生素残留的方法".食品科学 33.18(2012):206-210.