干旱区生态环境知识资源中心(Arid): 高效液相色谱-串联质谱法测定动物源食品中喹乙醇代谢物残留量

Arid

	高效液相色谱-串联质谱法测定动物源食品中喹乙醇代谢物残留量
其他题名	Determination of Olaquindox Metabolite Residues in Food Products of Animal Origin Using High Performance Liquid Chromatography-Tandem Mass Spectrometry
	刘正才 1; 杨方 1; 余孔捷 1; 林永辉 1; 李立 2
来源期刊	食品科学
ISSN	1002-6630
出版年	2012
卷号	33 期号:12 页码:210-214
中文摘要	建立动物源性食品中喹乙醇代谢残留标识物3-甲基喹喔啉-2-羧酸残留量的高效液相色谱串联质谱法。样品均质后,经Protease蛋白酶进行过夜酶解,加入盐酸酸化,离心,过滤后采用阴离子交换固相萃取柱Oasis MAX进行净化和富集。分析样品经Kinetex C_(18)色谱柱分离,在高效液相色谱-串联质谱多反应监测模式下进行定性、定量分析,采用正离子扫描。6种测定样品中3-甲基喹喔啉-2-羧酸定量下限均为0.5mug/kg,鳗鱼、虾、鸡肉、猪肉、牛肉和猪肝在0.5、1.0、4.0mug/kg和10.0mug/kg四个添加水平的平均回收率在60.7%~107%之间,相对标准偏差(n=10)在4.59%~14.9%之间。本方法分析速度快、灵敏度高、重现性好,各项技术指标均满足国内外相关法规要求,适用于动物源性食品中喹乙醇代谢物3-甲基喹喔啉-2-羧酸残留的确证检测。
英文摘要	A high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) method was developed to determine the olaquindox metabolite marker 3-methyl-quinoxaline-2-carboxylic acid(MQCA) in food products of animal origin.Samples were homogenized,hydrolyzed with protease overnight,acidified by adding hydrochloric acid,centrifugated,filtrated,and then cleaned up and enriched using an Oasis MAX solid phase extraction cartridge.The chromatographic separation was performed on a Kinetex C_(18) column.The qualitative and quantitative analysis of MQCA was carried out using an HPLC-MS/MS system under positive ion multiple reaction monitoring(MRM) mode.The limits of quantification for MQCA in meats from 6 different animal species(eel,shrimp,chicken,pork,beef and porcine liver) were all 0.5mug/kg.The average recoveries at four different spike levels: 0.5,1.0,4.0mug/mg and 10.0mug/kg were 60.7%-107% with relative standard deviation between 4.59% and 14.9%(n = 10).This method was fast,sensitive and reproducible and all of its technical indexes could meet the requirements of domestic and international relevant standards.Therefore it is applicable for the determination and confirmation of MQCA residues in food products of animal origin.
中文关键词	高效液相色谱-串联质谱法 ; 喹乙醇 ; 3-甲基喹喔啉-2-羧酸 ; 动物源性食品
英文关键词	liquid chromatography-tandem mass spectrometry(LC-MS/MS) qlaquindox 3-methyl-quinoxaline-2-carboxylic acid(MQCA) food products of animal origin
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:4580423
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/228536
作者单位	1.福建出入境检验检疫局, 福州, 福建 350001, 中国; 2.中国检验检疫科学研究院, 北京 100123, 中国
推荐引用方式 GB/T 7714	刘正才,杨方,余孔捷,等. 高效液相色谱-串联质谱法测定动物源食品中喹乙醇代谢物残留量[J],2012,33(12):210-214.
APA	刘正才,杨方,余孔捷,林永辉,&李立.(2012).高效液相色谱-串联质谱法测定动物源食品中喹乙醇代谢物残留量.食品科学,33(12),210-214.
MLA	刘正才,et al."高效液相色谱-串联质谱法测定动物源食品中喹乙醇代谢物残留量".食品科学 33.12(2012):210-214.