干旱区生态环境知识资源中心(Arid): 超高效液相色谱-电喷雾串联四极杆质谱法同时测定配合饲料中29种兽药

Arid

	超高效液相色谱-电喷雾串联四极杆质谱法同时测定配合饲料中29种兽药
其他题名	Simultaneous determination of 29 veterinary drugs in compound feeds by ultra performance liquid chromatography-electrospray ionization tandem quadrupole mass spectrometry
	赵颖; 刘瑜; 金雁; 徐宜宏; 钟钰; 蒋施; 李晓东; 曾凡; 周健南
来源期刊	色谱
ISSN	1000-8713
出版年	2012
卷号	30 期号:9 页码:908-914
中文摘要	建立了同时测定配合饲料中喹诺酮类、磺胺类、大环内酯类和硝基呋喃类共计29种兽药的超高效液相色谱-电喷雾串联四极杆质谱(UPLC-ESI-MS/MS)检测方法。饲料样品用甲醇-乙腈(1∶1,v/v)混合溶液提取,提取液经Oasis HLB固相萃取柱净化,采用UPLC-ESI-MS/MS检测。以甲醇和含0.1%(v/v)甲酸的水溶液作为流动相,进行梯度洗脱,用C_(18)色谱柱分离,正离子模式扫描,多反应监测模式检测。29种兽药在0.01~5.0 mg/L范围内线性关系良好,相关系数(r)均大于0.99;在复合预混饲料、全价配合饲料、浓缩饲料中4个添加水平下29种兽药的平均回收率在61.2%~94.3%范围内,相对标准偏差(RSD)为2.2%~15.0%;方法的检出限(以信噪比大于10计)为0.01 mg/kg或0.05 mg/kg。该方法简便、快速、准确,重现性好,灵敏度高,适用于配合饲料中多种兽药的同时检测。
英文摘要	An ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry(UPLC-ESI-MS/MS) method was developed for the simultaneous determination of 29 veterinary drugs of quinolones,sulfonamides,macrolides and nitrofurans in compound feeds.The sample was extracted with methanol-acetonitrile(1∶1,v/v),and then cleaned up using solid phase extraction with an Oasis HLB column,detected by UPLC-ESI-MS/MS.The UPLC separation was performed on a C_(18) column(100 mm*2.1 mm,1.7 mum) using a gradient elution with the mobile phases of methanol and 0.1%(v/v) formic acid aqueous solution.The ESI-MS/MS detection was achieved in positive mode under multiple reaction monitoring(MRM) mode.The linear ranges were from 0.01 to 5.0 mg/L with the correlation coefficients above 0.99 for all the 29 veterinary drugs.The average recoveries of the 29 veterinary drugs spiked in three feed matrixes(the compound premix feed,the complete formula feed and the concentrated feed) at four spiked levels were 61.2%-94.3% with the relative standard deviations(RSDs) of 2.2%-15.0%.The limits of detection were 0.01 mg/kg or 0.05 mg/kg.The method is rapid,accurate,reproducible,sensitive and easy to apply,and suitable for the simultaneous determination of diverse veterinary drugs in compound feeds.
中文关键词	超高效液相色谱-电喷雾串联四极杆质谱 ; 兽药 ; 配合饲料
英文关键词	ultra performance liquid chromatography-electrospray ionization tandem quadrupole mass spectrometry(UPLC-ESI-MS/MS) veterinary drugs compound feeds
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4640756
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/228390
作者单位	沈阳出入境检验检疫局综合技术中心, 沈阳, 辽宁 110016, 中国
推荐引用方式 GB/T 7714	赵颖,刘瑜,金雁,等. 超高效液相色谱-电喷雾串联四极杆质谱法同时测定配合饲料中29种兽药[J],2012,30(9):908-914.
APA	赵颖.,刘瑜.,金雁.,徐宜宏.,钟钰.,...&周健南.(2012).超高效液相色谱-电喷雾串联四极杆质谱法同时测定配合饲料中29种兽药.色谱,30(9),908-914.
MLA	赵颖,et al."超高效液相色谱-电喷雾串联四极杆质谱法同时测定配合饲料中29种兽药".色谱 30.9(2012):908-914.