Arid
超高效液相色谱-串联质谱法检测螺旋藻保健品中7种微囊藻毒素
其他题名Determination of 7 microcystins in Spirulina health food products by ultra performance liquid chromatography-tandem mass spectrometry
李兵; 刘伟; 范赛; 赵榕; 吴国华
来源期刊色谱
ISSN1000-8713
出版年2012
卷号30期号:6页码:584-589
中文摘要采用固相萃取净化结合超高效液相色谱-串联质谱法(UPLC-MS/MS)测定了螺旋藻保健品中7种微囊藻毒素(MCs)。螺旋藻保健品经70%(体积分数)的甲醇超声提取,冷冻离心沉淀杂质后,经HLB柱净化。在WatersACQUITY UPLC~? BEH C18色谱柱上以乙腈和0.2 mmo l/L乙酸铵水溶液为流动相进行梯度洗脱分离,采用电喷雾离子源正离子模式进行多反应离子监测,外标法定量。7种MC在线性范围内线性关系良好(相关系数(r)不小于0.995);检出限为6.7~33.3mug/kg,定量限为20.0~100.0mug/kg。各分析物在阴性螺旋藻保健品中的加标回收率在87.5%~97.9%之间,相对标准偏差(RSD)在1.6%~6.9%之间。该方法准确可靠,灵敏度高,可用于螺旋藻保健品中MC污染的确证定性、定量检测。
英文摘要Ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) combined with solid phase extraction(SPE) has been developed for the determination of 7 microcystins(MCs) in Spirulina health food products.The sample was extracted by 70%(v/v) methanol.The lipid substances were isolated by centrifugation under freezing condition,and then followed by clean-up with a Waters Oasis HLB solid phase extraction cartridge.The separation was performed on a Waters ACQUITY UPLCBEH C18 column with gradient elution using acetonitrile and 0.2 mmol/L ammonium acetate.The electrospray ionization(ESI) source in positive ion mode was used for multiple reaction monitoring(MRM).The external standard method was used for the quantification.The linear ranges for 7 MCs were 20-400 mug/kg,and the correlation coefficients were not less than 0.995.The limits of detection were 6.7-33.3 mug/kg.The limits of quantification were 20.0-100.0 mug/kg.The recoveries of the 7 MCs spiked in blank Spirulina samples ranged from 87.5% to 97.9% with the relative standard deviations of 1.6%-6.9%.The results demonstrated that the method is easy,fast,sensitive,and suitable for the confirmation and quantification of the 7 MCs in Spirulina samples.
中文关键词固相萃取 ; 超高效液相色谱-串联质谱 ; 微囊藻毒素 ; 螺旋藻保健品
英文关键词solid phase extraction(SPE) ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) microcystins(MCs) Spirulina health food products
语种中文
国家中国
收录类别CSCD
WOS类目CHEMISTRY ANALYTICAL
WOS研究方向Chemistry
CSCD记录号CSCD:4568728
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/228385
作者单位北京市疾病预防控制中心营养与食品卫生所, 北京 100013, 中国
推荐引用方式
GB/T 7714
李兵,刘伟,范赛,等. 超高效液相色谱-串联质谱法检测螺旋藻保健品中7种微囊藻毒素[J],2012,30(6):584-589.
APA 李兵,刘伟,范赛,赵榕,&吴国华.(2012).超高效液相色谱-串联质谱法检测螺旋藻保健品中7种微囊藻毒素.色谱,30(6),584-589.
MLA 李兵,et al."超高效液相色谱-串联质谱法检测螺旋藻保健品中7种微囊藻毒素".色谱 30.6(2012):584-589.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[李兵]的文章
[刘伟]的文章
[范赛]的文章
百度学术
百度学术中相似的文章
[李兵]的文章
[刘伟]的文章
[范赛]的文章
必应学术
必应学术中相似的文章
[李兵]的文章
[刘伟]的文章
[范赛]的文章
相关权益政策
暂无数据
收藏/分享

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。