干旱区生态环境知识资源中心(Arid): 超高效液相色谱-串联质谱法同时检测复合预混饲料中29种兽药

Arid

	超高效液相色谱-串联质谱法同时检测复合预混饲料中29种兽药
其他题名	Determination of 29 veterinary drugs in the compound premix feed by ultra performance liquid chromatography-tandem mass spectrometry
	刘瑜; 赵颖; 张鹏远; 金雁; 蒋施; 李晓东; 徐宜宏; 钟钰; 于丽; 周健南
来源期刊	分析试验室
ISSN	1000-0720
出版年	2012
卷号	31 期号:10 页码:77-81
中文摘要	建立了复合预混饲料中29种兽药同时检测的超高效液相色谱-串联质谱分析方法。样品经甲醇-乙腈(1:1,V/V)混合溶液提取,提取液经Oasis HLB固相萃取柱净化,29种兽药经Waters Acquity UPLC BEH C_(18)色谱柱分离,以甲醇和0.1%甲酸水溶液为流动相进行梯度洗脱。电喷雾正离子(ESI~+)模式电离,多反应监测模式检测,内标法定量。结果表明:29种兽药在0.01~5.0mug/mL范围内呈良好的线性关系,相关系数(r)均大于0.993;复合预混饲料中3个浓度添加水平平均回收率为65.5%~92.2%;相对标准偏差为3.8%~15%。
英文摘要	An ultra performance liquid chromatography-tandem mass spectrometric(UPLC-MS/MS)method was established for the determination of 29 veterinary drug.(6 quinolone drugs,14 sulfonamide drugs,3 nitrofuran drugs,6 macrolide drugs)residues in the compound premix feed.Samples were extracted with methanol-acetonitrile(50:50,V/V)solution.Extraction liquid was purified by an Oasis HLB SPE column.The separation of 29 veterinary drugs was performed on a Waters Acquity UPLC system with a BEH C_(18) column.The mobile phase was methanol and water(containing 0.1% formic acid)in gradient elution.The electrospray was operated in the positive ionization mode and the 29 veterinary drugs were identified by multiple reaction monitoring(MRM)mode.Internal standard method was adopted as the quantitative method.The calibration curves showed good linearity within the concentrations of 0.01~5.0 mug/mL with the correlation coefficients r>0.993.The recoveries of 29 veterinary drugs were 65.5%~92.2% at three spiked levels.The relative standard deviations were both less than 3.8%~15%.This method is simple,sensitive and accurate in the determination of veterinary drug residues.
中文关键词	超高效液相色谱-串联质谱 ; 兽药 ; 复合预混饲料
英文关键词	Ultra performance liquid chromatography-tandem mass spectrometry Veterinary drug Compound premix feed
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4642508
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/227939
作者单位	沈阳出入境检验检疫局, 沈阳, 辽宁 110016, 中国
推荐引用方式 GB/T 7714	刘瑜,赵颖,张鹏远,等. 超高效液相色谱-串联质谱法同时检测复合预混饲料中29种兽药[J],2012,31(10):77-81.
APA	刘瑜.,赵颖.,张鹏远.,金雁.,蒋施.,...&周健南.(2012).超高效液相色谱-串联质谱法同时检测复合预混饲料中29种兽药.分析试验室,31(10),77-81.
MLA	刘瑜,et al."超高效液相色谱-串联质谱法同时检测复合预混饲料中29种兽药".分析试验室 31.10(2012):77-81.