干旱区生态环境知识资源中心(Arid): 高效液相色谱-串联质谱法测定动物源食品中粘杆菌素、多粘菌素B的残留量

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	高效液相色谱-串联质谱法测定动物源食品中粘杆菌素、多粘菌素B的残留量
其他题名	Determination of colistin and polymyxin B residues in animal derived foods by high performance liquid chromatography tandem mass spectrometry
	苏萌 1; 艾连峰 2; 段文仲 2; 哈婧 1
来源期刊	分析试验室
ISSN	1000-0720
出版年	2012
卷号	31 期号:3 页码:73-77
中文摘要	建立了动物源食品中粘杆菌素和多粘菌素B残留的高效液相色谱-串联质谱(HPLC-MS/MS)测定方法。样品用V(10%三氯乙酸水溶液):V(乙腈)=30:70提取,Oasis WCX SPE柱净化,LC-MS/MS电喷雾正离子多反应监测模式(ESI~+-MRM)检测。分析物在0~250mug/kg的浓度范围内呈良好线性,线性相关系数>0.995。方法的定量限为10mug/kg。方法在三个添加水平的平均回收率在71.6%~78.9%之间,相对标准偏差在6.2%~12%之间。方法适用于动物源食品中粘杆菌素和多粘菌素B的定量及确证检测。
英文摘要	A sensitive liquid chromatography-tandem mass spectrometric method(LC-MS/MS) was developed to determine colistin and polymyxin B residues in animal derived foods.Samples were extracted with V(10% trichloroacetic acid solution): V(acetonitrile)=30:70 and purified by an Oasis WCX SPE column.LC-MS/MS analysis was performed on multiple reaction monitoring mode under positive ion electrospray ionization.The linearity is satisfactory with a correlation coefficient of >0.995 at concentrations ranging from 0 to 250 mug/kg.The limit of quantization(LOQ) is 10 mug/kg.Average recoveries of the analytes fortified at three level ranged from 71.6% to 78.9%,and the relative standard deviations ranged from 6.2% to 12%.It showed to be suitable for quantitative analysis and confirmation of t colistin and polymyxin B residues in animal derived foods.
中文关键词	高效液相色谱-串联质谱法 ; 粘杆菌素 ; 多粘菌素B ; 动物源食品
英文关键词	High performance liquid chromatography tandem mass spectrometry Colistin PolymyxinB Animal derived food
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4459424
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/227937
作者单位	1.河北科技大学化学与制药工程学院, 石家庄, 河北 050051, 中国; 2.河北出入境检验检疫局, 石家庄, 河北 050051, 中国
推荐引用方式 GB/T 7714	苏萌,艾连峰,段文仲,等. 高效液相色谱-串联质谱法测定动物源食品中粘杆菌素、多粘菌素B的残留量[J],2012,31(3):73-77.
APA	苏萌,艾连峰,段文仲,&哈婧.(2012).高效液相色谱-串联质谱法测定动物源食品中粘杆菌素、多粘菌素B的残留量.分析试验室,31(3),73-77.
MLA	苏萌,et al."高效液相色谱-串联质谱法测定动物源食品中粘杆菌素、多粘菌素B的残留量".分析试验室 31.3(2012):73-77.