干旱区生态环境知识资源中心(Arid): 液相色谱串联质谱法测定化妆品中的双酚A、辛基酚及壬基酚

Arid

	液相色谱串联质谱法测定化妆品中的双酚A、辛基酚及壬基酚
其他题名	Simultaneous Determination of Bisphenol A,Octylphenol and Nonylphenol in Skin Care Cosmetics by Liquid Chromatography-Tandem Mass Spectrometry
	郭新东; 冼燕萍; 陈立伟; 吴文海; 罗海英; 吴玉銮
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2012
卷号	31 期号:7 页码:848-852
中文摘要	建立了护肤化妆品中双酚A、辛基酚和壬基酚的超高效液相色谱串联质谱分析方法。样品经二氯甲烷提取,氨基固相萃取柱净化后,用甲醇定容,采用Waters UPLC BEH C_(18)色谱柱,以甲醇-0.05%氨水为流动相,梯度洗脱分离后,串联四极杆质谱多反应监测方式检测,以保留时间和子离子比定性,外标法定量。在优化条件下,该方法 5 min内可完成4种待测物的分析。双酚A、辛基酚和壬基酚在0.5~50.0mug/L范围内线性关系良好,相关系数均大于0.999,方法的定量下限(LOQs)均为0.5mug/kg;在加标水平为0.5、1.0、10.0mug/kg时,护肤霜和护肤水样品中4种待测物的平均加标回收率为80%~96%,相对标准偏差(n=6)小于15%。该方法准确、快速、灵敏,适用于护肤化妆品中双酚A、辛基酚和壬基酚的快速确认和定量检测。
英文摘要	A comprehensive analytical method for the determination of bisphenol A,octylphenol and nonylphenol in skin care cosmetics was developed using ultra performance liquid chromatography with tandem mass spectrometry(UPLC-MS/MS).The homogenized sample was extracted with methylene chloride.Sample concentration and purification were performed on an OASIS NH_2 solid phase extraction cartridge.The separation of 4 compounds was carried out on a Waters UPLC BEH C_(18) column by gradient elution with methanol-0.5% NH3·H2O as mobile phase.The analytes were detected under multiple reaction monitoring(MRM) with electrospry ionization in negative ion mode(ESI-),identified with retention time and ion ratio,and quantified by the external standard method.Under the optimal conditions,the separation of analytes was performed within 5 min.The calibration curves for four compounds were linear in the range of 0.5-50.0 mug/L with correlation coefficients more than 0.999.The quantitation limits were 0.5 mug/kg.The mean recoveries for four compounds in skin water and skin cream samples at three spiked concentration levels of 0.5,1.0,10.0 mug/kg were in the range of 80%-96% with RSDs(n=6) less than 15%.This method is accurate,simple and rapid,and is suitable for the inspection of bisphenol A,octylphenol and nonylphenol in skin care cosmetics.
中文关键词	护肤化妆品 ; 固相萃取 ; 液相色谱串联质谱法 ; 双酚A ; 辛基酚 ; 壬基酚
英文关键词	skin care cosmetics SPE UPLC-MS/MS bisphenol A octylphenol nonylphenol
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4596307
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/227933
作者单位	广州市质量监督检测研究院国家化妆品质量监督检验中心(广州), 广州, 广东 510110, 中国
推荐引用方式 GB/T 7714	郭新东,冼燕萍,陈立伟,等. 液相色谱串联质谱法测定化妆品中的双酚A、辛基酚及壬基酚[J],2012,31(7):848-852.
APA	郭新东,冼燕萍,陈立伟,吴文海,罗海英,&吴玉銮.(2012).液相色谱串联质谱法测定化妆品中的双酚A、辛基酚及壬基酚.分析测试学报,31(7),848-852.
MLA	郭新东,et al."液相色谱串联质谱法测定化妆品中的双酚A、辛基酚及壬基酚".分析测试学报 31.7(2012):848-852.