干旱区生态环境知识资源中心(Arid): 应用SPE柱净化HPLC/UV法测定乳制品及其原料中的硫氰酸盐

Arid

	应用SPE柱净化HPLC/UV法测定乳制品及其原料中的硫氰酸盐
其他题名	Determination of Thiocyanate in Milk Products and Raw Materials by HPLC/UV with SPE Extraction
	陶保华 1; 赖世云 1; 傅士姗 1; 蔡增轩 2; 张京顺 3; 董亦波 1; 任一平 2
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2012
卷号	31 期号:6 页码:686-690
中文摘要	采用SPE柱净化、高效液相反相离子对色谱分离、紫外检测器(HPLC/UV)测定乳制品及其原料中的硫氰酸盐。试样经乙酸沉淀蛋白、Oasis WAX SPE柱净化和浓缩,以甲醇和磷酸盐缓冲液加离子对试剂为流动相,经Symmetry C_(18)色谱柱分离,在231 nm波长处进行检测,外标法定量。结果表明,硫氰酸盐浓度在0.2~50 mg/L范围内线性良好,相关系数大于0.999,方法检出限为0.05 mg/kg,定量下限为0.15 mg/kg,在加标量为1、5、25 mg/kg时的回收率为77%~104%。该方法重现性好、检测时间短,灵敏度高,为乳制品及其原料中硫氰酸盐的定量检测提供了较为理想的方法。
英文摘要	A high performance liquid chromatography with tunable ultraviolet method(HPLC-UV) was developed for the quantification of thiocyanate in milk products and raw materials.Samples were purified and extracted by Oasis WAX SPEs followed by protein precipitation with acetic acid.The purified solution was separated on a symmetry C_(18) column using methanol-phosphatic solution(containing ion pair reagent) as mobile phase,and analyzed by the internal standard method at a detection wavelength of 231 nm.Under the optimal conditions,the linear range for thiocyanate was in the range of 0.2-50 mg/L with correlation coefficient more than 0.999.The limit of detection for thiocyanate was 0.05 mg/kg,and the limit of quantitation(LOQ) was 0.15 mg/kg.The recoveries at spiked concentration levels of 1,5,25 mg/kg were in the range of 77%-104%.With the advantages of excellent reproducibility,high sensitivity and good practicability,this method could be used as a routine detection in practice.
中文关键词	硫氰酸盐 ; SPE净化 ; 高效液相色谱 ; 紫外检测 ; 乳制品
英文关键词	thiocyanate SPE extract HPLC ultraviolet detection milk product
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4576593
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/227932
作者单位	1.贝因美研究院, 杭州, 浙江 310057, 中国; 2.浙江省疾病预防控制中心, 杭州, 浙江 310051, 中国; 3.浙江大学生物系统工程与食品科学学院, 杭州, 浙江 310029, 中国
推荐引用方式 GB/T 7714	陶保华,赖世云,傅士姗,等. 应用SPE柱净化HPLC/UV法测定乳制品及其原料中的硫氰酸盐[J],2012,31(6):686-690.
APA	陶保华.,赖世云.,傅士姗.,蔡增轩.,张京顺.,...&任一平.(2012).应用SPE柱净化HPLC/UV法测定乳制品及其原料中的硫氰酸盐.分析测试学报,31(6),686-690.
MLA	陶保华,et al."应用SPE柱净化HPLC/UV法测定乳制品及其原料中的硫氰酸盐".分析测试学报 31.6(2012):686-690.