干旱区生态环境知识资源中心(Arid): 沙冬青胚胎晚期发生丰富蛋白基因序列及表达特性分析

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	沙冬青胚胎晚期发生丰富蛋白基因序列及表达特性分析
其他题名	Sequence analysis and expression pattern of AmLEA14 encoding a late embryogenesis abundant protein in Ammopiptanthus mongolicus
	师静; 刘美芹; 史军娜; 赵晓鑫; 卢存福; 尹伟伦
来源期刊	北京林业大学学报
ISSN	1000-1522
出版年	2012
卷号	34 期号:4 页码:114-119
中文摘要	从沙冬青叶片EST文库中获得了胚胎晚期发生丰富蛋白(LEA)基因cDNA全长序列,命名为AmLEA14。序列分析表明该基因全长579bp,含有一个459bp编码152个氨基酸的开放阅读框,推测编码的蛋白质分子质量为16.6ku。二级结构预测显示此编码蛋白属于第4类LEA蛋白。系统发生分析表明,此编码蛋白与大豆LEA14蛋白(P46519.1)的亲缘关系最近。荧光定量PCR结果显示,AmLEA14的表达量在低温、干旱、盐胁迫条件下升高,但是主要在低温胁迫后期富集。推测AmLEA14基因可能在沙冬青抵御低温、干旱、盐胁迫中发挥作用,主要是参与沙冬青的低温防御机制。
英文摘要	For a better understanding of stress responses of desert plant Ammopiptanthus mongolicus,we reported molecular characterization and expression analysis of AmLEA14 gene from the EST data of cold-and drought-stressed A.mongolicus.Sequence analysis showed that the overall length of gene AmLEA14 was 579 bp,and the cDNA of AmLEA14 contained a 459 bp ORF encoding a polypeptide of 152 amio acids with a calculated molecular mass of 16.6 kDa and a theoretical pI of 4.9.Sequence and structural analysis showed that AmLEA14 protein was a member of late embryogenesis abundant protein class 4.Phylogenetic analysis indicated that AmLEA14 protein was most closely related to soybean LEA14 protein (P46519.1).Real-time quantitative PCR analysis showed that AmLEA14 was constitutively expressed,up-regulated by cold,drought stressed,and specifically accumulated at the late stage of cold treatment.Otherwise,AmLEA14 was down-regulated by heat stress.The combined results indicate that AmLEA14 protein plays some role in abiotic stress responses,mainly in cold tolerant mechanism,and is valuable for future abiotic stress researches in A.mongolicus.
中文关键词	沙冬青 ; 胚胎晚期发生丰富蛋白 ; 序列分析 ; 表达分析
英文关键词	Ammopiptanthus mongolicus late embryogenesis abundant protein sequence analysis expression analysis
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FORESTRY
WOS研究方向	Forestry
CSCD记录号	CSCD:4613385
来源机构	北京林业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/227703
作者单位	北京林业大学, 林木育种国家工程实验室;;教育部林木花卉遗传育种重点实验室, 100083
推荐引用方式 GB/T 7714	师静,刘美芹,史军娜,等. 沙冬青胚胎晚期发生丰富蛋白基因序列及表达特性分析[J]. 北京林业大学,2012,34(4):114-119.
APA	师静,刘美芹,史军娜,赵晓鑫,卢存福,&尹伟伦.(2012).沙冬青胚胎晚期发生丰富蛋白基因序列及表达特性分析.北京林业大学学报,34(4),114-119.
MLA	师静,et al."沙冬青胚胎晚期发生丰富蛋白基因序列及表达特性分析".北京林业大学学报 34.4(2012):114-119.