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新疆沙尘暴源区塔克拉玛干空气真菌群落的T-RFLP分析
其他题名Analysis on Fungal Diversity of Air Microbial Communities from Sandstorm Source Areas of the Taklamakan Desert in Xinjiang
段魏魏1; 娄恺2; 曾军2; 胡蓉1; 史应武2; 何清3; 刘新春3; 孙建2; 晁群芳1
来源期刊新疆农业科学
ISSN1001-4330
出版年2011
卷号48期号:4页码:769-775
中文摘要【目的】了解新疆塔克拉玛干沙漠周边地区空气真菌物种多样性及群落结构特征。【方法】应用末端限制性片段长度多态性(T-RFLP)技术分析和比较塔克拉玛干沙漠空气真菌多样性。【结果】塔克拉玛干沙漠周边且末地区的空气真菌物种多样性最高,尉犁地区最少,9个地区空气样品间相似性介于8.3%~41.9%,其中若羌和莎车地区的相似性最高,达到41.9%;尉犁地区与且末、叶城的相似性最低,为8.3%,与聚类分析结果大体上相一致。典型性相关分析(CCA)表明非生物环境因子(海拔、温度、风速等)影响空气真菌群落的多样性。【结论】塔克拉玛干沙漠周边9个地区空气真菌多样性较丰富,且各地区的优势真菌种群和丰度不同;限制性内切酶的种类对空气真菌群落信息的描述有较大影响。
英文摘要[Objective]The aim of the test was to reveal the air fungal diversity and compare the characteristics and the differences of air fungal communities in different regions around the edge of Taklamakan desert.[Method] Terminal-restriction fragment length polymorphism (T-RFLP)was used to analyze and compare fungal diversity of air microbial communities from 9 regions of the Taklamakan Desert in Xinjiang.[Result]The diversity index based on T-RFLP fingerprint showed the air fungal communites were rich in the Taklamakan Desert,Of them the highest Shannon index exists in Qarqan and the lowest was in Yuli.The similarity coefficient indicated the air fungal community similarity among 9 samples was between 8.3%-41.9%,Ruoqiang and Shache had the highest similarity in all (41.9%)and Yuli,Qarqan and Kargilik were the lowest(8.3%).Hierarchical Cluster Analysis had the same results with the similarity coefficient.Quantitative study showed that 9 regions of each species abundances was greatly different and had difference between dominant species and endemic species.The typical corresponding analysis (CCA)showed that the diversity index was highly affected by some environmental factors,such as altitude,temperature and wind speed.[Conclusion]The results suggested that the air fungal community was abundant from T-RFs fingerprint,and it is distinct between fungal species and abundance in different regions; the description of air microbial community was highly impacted by the choice of restriction enzymes.
中文关键词塔克拉玛干沙漠 ; 空气真菌多样性 ; 沙尘暴
英文关键词T-RFLP the Taklamakan Desert T-RFLP air fungal diversity sandstorm
语种中文
国家中国
收录类别CSCD
WOS类目AGRICULTURE MULTIDISCIPLINARY
WOS研究方向Agriculture
CSCD记录号CSCD:4207284
来源机构新疆大学 ; 中国气象局乌鲁木齐沙漠气象研究所
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/227275
作者单位1.新疆大学生命科学与技术学院, 乌鲁木齐, 新疆 830046, 中国;
2.新疆特殊环境微生物实验室, 乌鲁木齐, 新疆 830091, 中国;
3.中国气象局乌鲁木齐沙漠气象研究所, 乌鲁木齐, 新疆 830002, 中国
推荐引用方式
GB/T 7714
段魏魏,娄恺,曾军,等. 新疆沙尘暴源区塔克拉玛干空气真菌群落的T-RFLP分析[J]. 新疆大学, 中国气象局乌鲁木齐沙漠气象研究所,2011,48(4):769-775.
APA 段魏魏.,娄恺.,曾军.,胡蓉.,史应武.,...&晁群芳.(2011).新疆沙尘暴源区塔克拉玛干空气真菌群落的T-RFLP分析.新疆农业科学,48(4),769-775.
MLA 段魏魏,et al."新疆沙尘暴源区塔克拉玛干空气真菌群落的T-RFLP分析".新疆农业科学 48.4(2011):769-775.
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