干旱区生态环境知识资源中心(Arid): 失水-吸水过程中集球藻的生理生化特性研究

Arid

	失水-吸水过程中集球藻的生理生化特性研究
其他题名	STUDY ON THE PHYSIOLOGICAL AND BIOCHEMICAL PROPERTIES OF PALMELLOCOCCUS SP. SUBJECTED TO DEHYDRATION AND SUBSEQUENT REHYDRATION
	饶本强; 李敦海; 吴沛沛; 沈银武; 刘永定
来源期刊	水生生物学报
ISSN	1000-3207
出版年	2011
卷号	35 期号:2 页码:340-347
中文摘要	荒漠结皮藻类广泛分布于干旱、半干旱地区,经常面临生存水源的短缺。以一种从荒漠结皮中分离的典型绿藻-集球藻(Palmellococcus sp.)为材料,研究其在失水-吸水过程中的某些生理生化特性。即在温室模拟条件下设置相对湿度分别为0%(极度失水)、43%(适度失水)和98%(水合,对照),测定不同失水处理对集球藻光合活性、膜脂过氧化产物丙二醛含量、细胞内可溶性物质含量和抗氧化酶活性的影响,并测定蒸馏水、BBM培养基、0.2 g/L蔗糖溶液、0.2 g/L蓝藻多糖、0.2 g/L脯氨酸和2.5 mmol/L氯霉素及50mumol/L敌草隆进行吸水处理时对失水藻体光合活性的恢复效果。结果表明,与对照处理相比,集球藻在失水过程中光合活性迅速降低,SOD和CAT活性大量升高,细胞内可溶性糖含量和可溶性蛋白含量明显增加,并且膜脂丙二醛含量出现大量积累。不同的失水-吸水处理发现,外源蔗糖和胞外多糖吸水对集球藻的光合活性有较好的恢复作用,添加脯氨酸和氯霉素吸水时光合活性也获得了一定程度的恢复,采用敌草隆吸水时则出现光合活性的明显抑制。研究结果对于更好地理解荒漠结皮绿藻对干旱的生理适应机制提供了有意义的参考。
英文摘要	Desert algae have a cosmopolitan distribution in arid and semi-arid regions, and are frequently subjected to absence of water source for survival demands.They are poikilohydric organisms whose lifestyle in many cases consists of alternating periods of drought.A green algae Palmellococcus sp., isolated from desert algal crusts, is apparently sub-jected to such extreme transitions between dehydration and rehydration.In this study, the effect of dehydration and subsequent rehydration on PSⅡ activity(Fv/Fm), MDA contents and intracellular production of water-soluble substances as well as antioxidant enzyme activities were investigated for Palmellococcus sp.in laboratory.Concerning the experi-ments, three drought treatments at different relative humidity(RH) were designed as follows:RH= 0%(exceedingly droughty), RH=43%(moderately droughty) and RH=98%(fully hydrated, as control).Subsequently, rehydration were conducted by using of BBM medium, extracellular polysaccharides(EPS, 0.2 g/L), sucrose solution(0.2 g/L), proline solution(0.2 g/L) and distilled water as well as 2.5mM CMP and 50muM DCMU.The results showed that drought re-sulted in a rapid decrease in PSⅡ activity of Palmellococcus sp.and increased antioxidant enzyme activities of catalase(CAT, EC 1.11.1.6) and superoxide dismutase(SOD, EC 1.15.1.1) when compared to the control treatment.It also indicated that intracellular production of water-soluble substances such as saccharides and proteins were comparatively greater in the dehydrated cells than the control cells, and simultaneously drought caused a significantly deleterious effect on membrane lipid superoxide as assessed by measurement of MDA content.In addition, we found that hydration induced an apparent increase in PSⅡ activity of Palmellococcus sp., and the roles of exterior exopolysaccharide(EPS) and sucrose in photosynthetic recovery were more effective than other rehydration treatments.Research results may provide us a significant finding for better understanding the tolerance mechanism of desert green algae to drought environment.
中文关键词	结皮绿藻 ; 集球藻 ; 失水-吸水 ; 光合活性 ; 细胞可溶性物质 ; 抗氧化酶活性 ; 膜脂过氧化
英文关键词	Crust green algae Palmellococcus sp. Dehydration Rehydration PSⅡ activity Intracellular wa-ter-soluble substances Antioxidant enzyme activities Membrane lipid superoxid
类型	Article
语种	中文
国家	中国
收录类别	CSCD
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
CSCD记录号	CSCD:4384270
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/227043
作者单位	饶本强, 中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉, 湖北 430072, 中国.; 李敦海, 中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉, 湖北 430072, 中国.; 吴沛沛, 中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉, 湖北 430072, 中国.; 沈银武, 中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉, 湖北 430072, 中国.; 刘永定, 中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉, 湖北 430072, 中国.
推荐引用方式 GB/T 7714	饶本强,李敦海,吴沛沛,等. 失水-吸水过程中集球藻的生理生化特性研究[J],2011,35(2):340-347.
APA	饶本强,李敦海,吴沛沛,沈银武,&刘永定.(2011).失水-吸水过程中集球藻的生理生化特性研究.水生生物学报,35(2),340-347.
MLA	饶本强,et al."失水-吸水过程中集球藻的生理生化特性研究".水生生物学报 35.2(2011):340-347.