干旱区生态环境知识资源中心(Arid): 固相萃取-超高效液相色谱-质谱/质谱法测定动物源性食品中二硝甲酚残留量

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	固相萃取-超高效液相色谱-质谱/质谱法测定动物源性食品中二硝甲酚残留量
其他题名	Determination of 4,6-Dinitro-O-Cresol(DNOC)Residue in Animal-origin Foods by Solid-phase Extraction and Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry
	吴岩 1; 康庆贺 1; 刘永 1; 胡珅 2; 杨长志 1; 高凯洋 3
来源期刊	食品科学
ISSN	1002-6630
出版年	2011
卷号	32 期号:14 页码:268-272
中文摘要	建立动物源性食品中二硝甲酚残留量的分析方法。样品以丙酮-正己烷(1:2,V/V)为提取溶剂,经高速匀浆方法提取,提取液经Oasis HLB固相萃取柱净化,除去样品中大部分的脂肪和无机盐等干扰基质,有效地降低了样品中的复杂基质所带来的背景干扰。以乙腈-水(梯度洗脱)为流动相经BEH C18柱(50mm*2.1mm,1.7mum)进行分离后以MS/MS多反应监测扫描模式下检测。方法的相关系数r＞0.999,最低检出限为0.005mg/kg。在3种添加水平(0.005、0.010、0.020mg/kg)条件下,其平均回收率为82.6%～108%,相对标准偏差小于10%(n＝6)。该方法是一种快速、准确、灵敏度高的检测动物源性食品中二硝甲酚残留量的检测方法
英文摘要	A solid-phase extraction and ultrahigh performance liquid chromatography-tandem mass spectrometry method has been developed for the determination of DNOC residue in animal-origin foods.Samples were extracted with acetone-hexane(1:2,V/V)after high-speed homogenization.The crude extract was purified on Oasis HLB solid phase extraction column to remove fat and inorganic salts,which could effectively reduce the background interference generated by complex matrix in samples.The DNOC residue was separated on BEH C18(50 mm*2.1 mm,1.7mum)column using acetonitrile-water as the mobile phase by gradient elution and detected using a tandem mass analyzer in the multi-reaction monitoring(MRM)mode.The developed method exhibited an excellent linear relationship(r＞0.999)and a detection limit of 0.005 mg/kg.At the spiked levels of 0.005,0.01 mg/kg and 0.02 mg/kg,the average recovery rates for DNOC were in the range of 82.6%-108%with a relative standard deviation(RSD)of less than 10%(n=6).This method proved rapid,accurate and highly sensitive and can therefore be used for the determination of DNOC residue in animal-origin foods.
中文关键词	固相萃取 ; 超高效液相色谱-质谱/质谱 ; 二硝甲酚 ; 动物源性食品 ; 除草剂
英文关键词	solid-phase extraction(SPE) ultrahigh performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) DNOC animal-origin food herbicide
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FOOD SCIENCE TECHNOLOGY
WOS研究方向	Food Science & Technology
CSCD记录号	CSCD:4308315
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/227024
作者单位	1.黑龙江出入境检验检疫局, 哈尔滨, 黑龙江 150001, 中国; 2.东宁出入境检验检疫局, 东宁, 黑龙江 157200, 中国; 3.黑龙江大学化学化工与材料学院, 哈尔滨, 黑龙江 150086, 中国
推荐引用方式 GB/T 7714	吴岩,康庆贺,刘永,等. 固相萃取-超高效液相色谱-质谱/质谱法测定动物源性食品中二硝甲酚残留量[J],2011,32(14):268-272.
APA	吴岩,康庆贺,刘永,胡珅,杨长志,&高凯洋.(2011).固相萃取-超高效液相色谱-质谱/质谱法测定动物源性食品中二硝甲酚残留量.食品科学,32(14),268-272.
MLA	吴岩,et al."固相萃取-超高效液相色谱-质谱/质谱法测定动物源性食品中二硝甲酚残留量".食品科学 32.14(2011):268-272.