干旱区生态环境知识资源中心(Arid): 3种黑杨无性系水分利用效率差异性分析及相关ERECTA基因的克隆与表达

Arid

	3种黑杨无性系水分利用效率差异性分析及相关ERECTA基因的克隆与表达
其他题名	The differences of water use efficiency (WUE)among three Populus deltoids clones,and the cloning and characterization of related gene,PdERECTA
	郭鹏; 夏新莉; 尹伟伦
来源期刊	生态学报
ISSN	1000-0933
出版年	2011
卷号	31 期号:11 页码:3239-3245
中文摘要	提高植物水分利用效率(wuE)是未来解决我国甚至世界干旱缺水的最重要手段之一。因此,培育和筛选高wuE的品系和品种意义重大。通过对3种在充分浇水处理下不同北美黑杨无性系WUE、叶片delta~(13)C的差异分析,发现wuE在黑杨无性系之间也存在差异。ERECTA基因可以通过改变气孔密度来提高WUE。在欧美杨中克隆到了该基因(PdERECTA)。该基因序列长 2844bp,编码948个氨基酸,并且具有保守的类受体蛋白激酶家族的膜外富含亮氨酸和胞内激酶区域。荧光定量表达分析证明该基因在ABA、盐、冷等胁迫处理下都被诱导表达。组织特异表达分析说明PdERECTA基因在顶端叶表达量最高,半成熟叶次之,成熟叶衰老叶和根中则元表达。
英文摘要	In the many parts of the world where water is in short supply,plant water use efficiency,the ratio of carbon fixation to water loss,is critical to plant survival,crop yield and vegetation dynamics.So much effort is being made to reduce water use by crops and produce more crops per drop.Promoting plant water use efficiency is one of the most significant strategies to save water.For the evidence of variation in WUE among species,cultivars and populations.Therefore,it is of great significance to cultivate and choose good WUE clone.Populus deltoides is one of the most important species for largescale forestation projects in china.However,the trait of high- consumption-water becomes the key limited factor in the arid and semi-arid areas In our test,Carbon isotope discrimination (delta~(13)C)is a measure of the ~(13)C/~(12)C ratio in plant material,and is positively correlated with WUE.We therefore examined delta~(13)C to assess WUE in our study,the average ~(13)C/~(12)C of NE-19,R-270 and DN-2 was -29.732,-30.758 and -31.606,respectively.For farmers and agronomists,the unit of production (WUEL)is much more likely to be the yield of harvested product achieved from the water made available to the crop through rainfall or irrigation.In our study,we found that the WUEL of NE-19,R-270 and DN-2 was 2.41,2.12 and 1.98 mg/ml,respectively.Although WUE can be studied by many strategies,little is known about the genetic control of transpiration efficiency Our interest has therefore focused on identifying genes whose function could increases plant water use efficiency (WUE).Thus,finding new key genes responsible for water use efficiency phenotypes is of great importance not only for a better understanding of stress responses,but also for promising future crop improvement.The ERECTA gene regulates plant transpiration efficiency in Arabidopsis.In our paper,A cDNA clone,designated PdERECTA,was isolated from Populus deltoids.And the cDNA of PdERECTA was 2844bp and contained a single open reading frame of 948 amino acid residues.The deduced amino acid sequence of PdERECTA shows characteristics of a transmembrane receptor protein kinase with distinct domains:extracellular domain; transmembrane domain; intracellular kinase domain.To determine which type of stress could induce PdERECTA expression,total RNA was extracted from One-year-old NE-19 treated with various stress.qRT-PCR indicated that mRNA accumulation of PdERECTA was induced by low temperatures ,salt stress,abscisic acid (ABA)Dehydration.In order to further determine the pattern of tissue specific expression of PdERECTA under well-water conditions,different organs of were harvested for RNA extraction.In the paper,we also found that PdERECTA was strongly expressed in top-leaves,weakly in immature-leaves but not in senescent- leaves and roots,indicating its function in normal plant growth and development.
中文关键词	ERECTA基因 ; 北美黑杨 ; 叶片delta~(13) ; 水分利用效率(WUE)
英文关键词	ERECTA gene Populus deltoids leaf delta~(13)C water use efficiency (WUE)
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FORESTRY
WOS研究方向	Forestry
CSCD记录号	CSCD:4219151
来源机构	北京林业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/226933
作者单位	北京林业大学, 林木育种国家工程实验室, 北京 100083, 中国
推荐引用方式 GB/T 7714	郭鹏,夏新莉,尹伟伦. 3种黑杨无性系水分利用效率差异性分析及相关ERECTA基因的克隆与表达[J]. 北京林业大学,2011,31(11):3239-3245.
APA	郭鹏,夏新莉,&尹伟伦.(2011).3种黑杨无性系水分利用效率差异性分析及相关ERECTA基因的克隆与表达.生态学报,31(11),3239-3245.
MLA	郭鹏,et al."3种黑杨无性系水分利用效率差异性分析及相关ERECTA基因的克隆与表达".生态学报 31.11(2011):3239-3245.