干旱区生态环境知识资源中心(Arid): 固相萃取-液相色谱-质谱/质谱法同时测定蜂蜜中的多类药物残留

Arid

	固相萃取-液相色谱-质谱/质谱法同时测定蜂蜜中的多类药物残留
其他题名	Simultaneous determination of multi-veterinary drug residues in honey by solid phase extraction-high performance liquid chromatography-tandem mass spectrometry
	侯建波 1; 谢文 1; 陈笑梅 1; 奚君阳 2; 钱艳 2; 王峰 2; 刘海山 1
来源期刊	色谱
ISSN	1000-8713
出版年	2011
卷号	29 期号:6 页码:535-542
中文摘要	建立了采用固相萃取-液相色谱-质谱/质谱(SPE-LC-MS/MS)对蜂蜜中磺胺类、硝基咪唑类、喹诺酮类、大环内酯类、林可酰胺类和吡喹酮共计6大类54种药物残留同时测定的方法。蜂蜜经磷酸盐缓冲溶液(pH 8)稀释,Oasis HLB固相萃取柱净化后,通过液相色谱-质谱联用技术进行检测(正离子方式,多反应监测模式)。采用同位素稀释内标法或外标法进行定量,线性关系良好,相关系数大于0.992。方法的定量限(LOQ,以信噪比(S/N)大于10计)分别为磺胺类和硝基咪唑类药物1.0mug/kg,喹诺酮类和林可酰胺类药物2.0mug/kg,大环内酯类药物3.0mug/kg,吡喹酮0.3mug/kg。总体回收率为32.6%～114%,相对标准偏差为1.3%～28.9%。该方法的定量限满足目前国内外药物的最大残留限量要求,可作为蜂蜜中相关药物残留量的筛选检测方法。
英文摘要	A method of solid phase extraction-liquid chromatography-tandem mass spectrometry(SPE-LC-MS/MS)for simultaneous determination of 54 drug residues(sulfonamides,nitroimidazoles,quinolones,macrolides,lincosamides and praziquantel)in honey was developed.The honey samples were diluted by phosphate buffer solution(pH 8)followed by a further cleanup procedure with an Oasis(HLB)SPE column.The detection was carried out by LC-MS/MS,using positive-ion electrospray ionization in multiple reaction monitoring(MRM)mode,with one precursor/two product ion transitions for each compound.Isotope dilution internal standard method or external standard method was used to determine the residue contents with a good linear relationship(r>0.992).The limits of quantification(LOQs,S/N>10)were 1.0 mug/kg for sulfonamides and nitroimidazoles,2.0 mug/kg for quinolones and lincosamides,3.0 mug/kg for macrolides,and 0.3 mug/kg for praziquantel.The recovery ranges were from 32.6% to 114% with the relative standard deviations (RSDs)between 1.3% and 28.9%.As a screening method,the developed procedure is suitable for the determination and confirmation of the drug residues in honey samples.
中文关键词	液相色谱-质谱/质谱 ; 多类药物残留 ; 蜂蜜
英文关键词	liquid chromatography-tandem mass spectrometry(LC-MS/MS) multi-veterinary drug residues honey
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4238391
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/226849
作者单位	1.浙江出入境检验检疫局技术中心, 杭州, 浙江 310016, 中国; 2.浙江立德产品技术有限公司, 杭州, 浙江 310016, 中国
推荐引用方式 GB/T 7714	侯建波,谢文,陈笑梅,等. 固相萃取-液相色谱-质谱/质谱法同时测定蜂蜜中的多类药物残留[J],2011,29(6):535-542.
APA	侯建波.,谢文.,陈笑梅.,奚君阳.,钱艳.,...&刘海山.(2011).固相萃取-液相色谱-质谱/质谱法同时测定蜂蜜中的多类药物残留.色谱,29(6),535-542.
MLA	侯建波,et al."固相萃取-液相色谱-质谱/质谱法同时测定蜂蜜中的多类药物残留".色谱 29.6(2011):535-542.