干旱区生态环境知识资源中心(Arid): 超高效液相色谱法同时测定化妆品中多种雌激素

Arid

	超高效液相色谱法同时测定化妆品中多种雌激素
其他题名	Determination of estrogens in cosmetics by ultrahigh performance liquid chromatography
	贾彦博; 孙岚; 屠海云; 王红青
来源期刊	日用化学工业
ISSN	1001-1803
出版年	2011
卷号	41 期号:4 页码:303-306
中文摘要	建立了化妆品中5种雌激素（己烯雌酚、17-alpha-雌二醇、17-beta-雌二醇、己烷雌酚、17-alpha-乙炔雌二醇）的超高效液相色谱（UPLC）测定方法。化妆品样品采用氯化钠饱和溶液分散,然后用乙腈从分散液中提取雌激素类药物,用亚铁氰化钾和醋酸锌从提取液中沉淀大分子,OasisHLB固相萃取小柱富集净化,以W aters ACQUITY UPLC BEH C18色谱柱（2.1 mm*100 mm,1.7mum）分离,经乙腈-水为流动相梯度洗脱,5 m in内完成5种雌激素的分离及检测。在0.58.0 mg/L进样质量浓度范围内,5种雌激素的工作曲线的线性相关系数均高于0.999 5;在0.10,0.20,0.50 mg/kg 3个添加水平下5种雌激素的平均回收率为83.1%95.4%,相对标准偏差小于7.4%。
英文摘要	A method was established for the determination of five estrogens（diethylstilbestrol,17-alpha-estradiol,17-beta-estradiol,dihydroethylstilbestrol and 17-alpha-ethinyl estradiol） in cosmetics by ultrahigh performance liquid chromatography（UPLC）.The cosmetic sample was firstly dispersed by saturated NaCl solution and then the dispersion was extracted with acetonitrile.The extract was treated by potassium ferrocyanide and zinc acetate to cause the precipitation of big molecular compounds.The clear phase was enriched and purified by a Oasis HLB solid phase extraction cartridge.Then the five estrogens were separated and detected within 5 min on a chromatograph column of Waters ACQUITY UPLC BEH C18（2.1 mm*100 mm,1.7 mum） by gradient elution with acetonitrile-water system.In the range of 0.58.0 mg/L,the calibration curves of five estrogens show good linearity with the correlation coefficients larger than 0.999 5.The mean recoveries at the three spike levels（0.10,0.20,0.50 mg/kg） were 83.1%95.4% with less than 7.4% of relative standard deviations.
中文关键词	化妆品 ; 雌激素 ; 超高效液相色谱法
英文关键词	cosmetics estrogen ultrahigh performance liquid chromatography
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENGINEERING CHEMICAL
WOS研究方向	Engineering
CSCD记录号	CSCD:4272363
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/226848
作者单位	杭州市质量技术监督检测院, 杭州, 浙江 310019, 中国
推荐引用方式 GB/T 7714	贾彦博,孙岚,屠海云,等. 超高效液相色谱法同时测定化妆品中多种雌激素[J],2011,41(4):303-306.
APA	贾彦博,孙岚,屠海云,&王红青.(2011).超高效液相色谱法同时测定化妆品中多种雌激素.日用化学工业,41(4),303-306.
MLA	贾彦博,et al."超高效液相色谱法同时测定化妆品中多种雌激素".日用化学工业 41.4(2011):303-306.