干旱区生态环境知识资源中心(Arid): 水中2,4-滴、灭草松和莠去津的高效液相色谱测定法

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	水中2,4-滴、灭草松和莠去津的高效液相色谱测定法
其他题名	Determination of 2,4-D,Bentazone and Atrazine in Water by High Performance Liquid Chromatography
	莫婉湫; 刘健明; 陈明; 巢猛
来源期刊	环境与健康杂志
ISSN	1001-5914
出版年	2011
卷号	28 期号:6 页码:532-534
中文摘要	目的建立了水中2,4-滴、灭草松和莠去津的固相萃取一高效液相色谱(SPE-HPLC)同时测定法。方法选用WatersSunfire~(TM)C18色谱柱(150mmx4.6mm,5mum),以磷酸盐缓冲溶液+乙腈作为流动相进行梯度洗脱,流量为0.5ml/min,柱温35℃,检测波长230nm。水样经Oasis?HLB3ccSPE柱进行固相萃取后进行高效液相色谱分析。结果在0.05-2.00mg/L线性范围内,所得2,4-滴、灭草松和莠去津的回归方程均呈较好的线性关系,相关系数>0.9995。该方法所得2,4-滴、灭草松和莠去津的检出限分别为0.1、0.2、0.2mug/L,平均回收率分别为 98.30%-99.400-/0.98.60%-101.40%和101.10%-102.000-/o,RSD<1.5%。结论该方法操作简便、快速,精密度和准确度均较好,分离效果理想,灵敏度高,适用于地表水和饮用水中2,4-滴、灭草松和莠去津的同时测定。
英文摘要	Objective To establish a solid extraction and high performance liquid chromatography method for simultaneous determination of 2,4-D,bentazone and atrazine in water.Methods The HPLC separation was conducted by using a Wates SunfireTM C18 column (150 mmx4.6 mm,5 mum).Mobile phase were phosphate buffer solution and acyanide,flow rate was 0.5 ml/min,column temperature was 35 ℃,the UV detector was used with wavelength of 230 nm.The samples were extracted by Oasis HLB 3cc SPE,and then determined by HPLC.Results There were good linear relationships of 2,4-D,bentazone and atrazine over the range of 0.05 to 2.00 mg/L,r>0.999 5,the limits of detection for 2,4-D,bentazone and atrazine were 0.1,0.2 and 0.2 mug/L.The average recovery rates were 98.30% to 99.40% for 2,4-D,98.60% to 101.40% for bentazone,and 101.10% to 102.00% for atrazine,with the relative standard deviations of below 1.5%.Conclusion The method is simple,rapid,accurate and is applicable to simultaneous determination of 2,4-D,bentazone and atrazine both in surface water and drinking water with high sensitivity and high selectivity.
中文关键词	色谱法,高效液相 ; 固相萃取 ; 2,4-滴 ; 灭草松 ; 莠去津 ; 水
英文关键词	Chromatography,high performance liquid Solid phase extraction 2,4-dichlorophenoxyacetic acid Bentazone Atrazine Water
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4278738
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/226738
作者单位	东莞市东江水务有限公司水质监测站, 东莞, 广东 523109, 中国
推荐引用方式 GB/T 7714	莫婉湫,刘健明,陈明,等. 水中2,4-滴、灭草松和莠去津的高效液相色谱测定法[J],2011,28(6):532-534.
APA	莫婉湫,刘健明,陈明,&巢猛.(2011).水中2,4-滴、灭草松和莠去津的高效液相色谱测定法.环境与健康杂志,28(6),532-534.
MLA	莫婉湫,et al."水中2,4-滴、灭草松和莠去津的高效液相色谱测定法".环境与健康杂志 28.6(2011):532-534.