干旱区生态环境知识资源中心(Arid): 固相萃取-高效液相色谱/串联质谱检测谷物中黄曲霉毒素B_1、B_2、G_1、G_2和M

Arid

	固相萃取-高效液相色谱/串联质谱检测谷物中黄曲霉毒素B_1、B_2、G_1、G_2和M_1
其他题名	Determination of aflatoxin B_1, B_2, G_1, G_2 and M_1 in corn by high performance liquid chromatography-tandem mass spectrometry
	王岩松 1; 范世华 2; 李华锋 1; 张凤清 1; 贺明睿 1; 崔相勇 1
来源期刊	分析试验室
ISSN	1000-0720
出版年	2011
卷号	30 期号:10 页码:63-67
中文摘要	建立了高效液相色谱/串联质谱(LC-MS/MS)同时检测谷物中的黄曲霉毒素B_{1、B_{2、G_{1、G_{2和M_{1的方法。并优化了液相色谱条件和质谱的相关参数。谷物样品经研磨成粉末后，直接经甲醇-水（V:V=10：90）提取，Oasis HLB固相萃取净化，乙腈-水（0.2%甲酸）梯度洗脱，选择电喷雾离子源（ESI），正离子扫描多反应监测（MRM）模式，外标法定量。黄曲霉毒素B_{1、B_{2、G_{1、G_{2和M_{1的定量下限分别为 0.1、0.1、0.2、0.3、0.2 ng/g，平均回收率在 74.6%～ 89.6%之间，测试精密度（RSD）在 5.2%～ 11.3%之间。方法已用于谷物样品检测，黄曲霉毒素残留量最高达到B_{1 5.86 ng/g 、G_{1 8.6 ng/g、M_{1 4.0 ng/g，B_{2和G_{2未检出。}}}}}}}}}}}}}}}
英文摘要	A sensitive method was developed for the determination of aflatoxin B_1, B_2, G_1, G_2 and M_1 in corn by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Analysis conditions of liquid chromatography and mass spectrometry were optimized. Aflatoxins were extracted with 10% (V/V) methanol-water after corn was grinded into powder, purified through an Oasis HLB cartridge, eluted by methanol and water containing 0.2% formic acid, and determinated by electrospray ionization in positive ion mode and using multiple reaction monitoring (MRM). The limits of quantification for aflatoxin B_1, B_2, G_1, G_2 and M_1 were 0.1 ng/g, 0.1 ng/g, 0.2 ng/g, 0.3 ng/g and 0.2 ng/g, the correlation coefficients were more than 0.9975 in respective linear ranges, and recoveries for analytes in different matrixs were 74.6% ～ 89.6% and the relative standard deviations (RSD) in different concentration levels were all less than 11.3%. The proposed method was sensitive to meet the requirements for monitoring aflatoxin B_1, B_2, G_1, G_2 and M1 in corn.
中文关键词	高效液相色谱-串联质谱 ; 固相萃取 ; 黄曲霉毒素B_1、B_2、G_1、G_2、M_1 ; 谷物
英文关键词	High Performance Liquid Chromatography-Tandem Mass Spectrometry Solid phase extraction Aflatoxin B_1, B_2, G_1, G_2 and M_1 Corn
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4355479
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/226384
作者单位	1.沈阳产品质量监督检验院, 沈阳, 辽宁 110022, 中国; 2.东北大学分析科学研究中心, 沈阳, 辽宁 110004, 中国
推荐引用方式 GB/T 7714	王岩松,范世华,李华锋,等. 固相萃取-高效液相色谱/串联质谱检测谷物中黄曲霉毒素B_1、B_2、G_1、G_2和M_1[J],2011,30(10):63-67.
APA	王岩松,范世华,李华锋,张凤清,贺明睿,&崔相勇.(2011).固相萃取-高效液相色谱/串联质谱检测谷物中黄曲霉毒素B_1、B_2、G_1、G_2和M_1.分析试验室,30(10),63-67.
MLA	王岩松,et al."固相萃取-高效液相色谱/串联质谱检测谷物中黄曲霉毒素B_1、B_2、G_1、G_2和M_1".分析试验室 30.10(2011):63-67.