干旱区生态环境知识资源中心(Arid): 液相色谱-三级质谱法分析尿液中beta2-受体激动剂及beta-受体阻断剂多组分残留

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	液相色谱-三级质谱法分析尿液中beta2-受体激动剂及beta-受体阻断剂多组分残留
其他题名	Determination of 12beta2-Agonists and 4beta-Blockers in Urine by High Performance Liquid Chromatography-ion Trap Mass Spectrometry
	范赛 1; 邹建宏 2; 苗虹 3; 吴永宁 1; 赵云峰 1
来源期刊	分析化学
ISSN	0253-3820
出版年	2011
卷号	39 期号:8 页码:1153-1158
中文摘要	建立了SPE柱净化结合液相色谱-三级质谱测定尿液中12种beta2-受体激动剂和4种beta-受体阻断剂的方法。尿液样品经冷冻离心,加入beta-葡萄糖苷酸酶酶解后,以高氯酸沉淀蛋白,经HLB和MCX固相萃取柱净化。在Waters Atlantis?T3色谱柱上以甲醇和含0.1%甲酸流动相进行梯度洗脱分离,采用ESI源正离子模式进行三级子离子监测,外标法定量。16种化合物的线性范围在0.005~0.160mg/kg之间,相关系数(r)大于0.995;各化合物检出限均低于0.2mug/kg。以0.005,0.01和0.02mg/L为添加水平的空白尿液的加标回收率在41.2%~88.2%之间;相对标准偏差在2.7%~19.2%之间。本方法准确可靠,灵敏度高,可用于尿液中beta2-受体激动剂和beta-受体阻断剂残留的定性与定量检测。
英文摘要	A high performance liquid chromatography-ion trap mass spectrometric method combined with the technique of solid phase extraction had been developed for the determination of 12beta2-agonists and 4beta-blockers in urine samples.Urine samples were enzyme hydrolyzed bybeta-glucuronidase/arylsulfatase,then extracted and deproteinized by perchloric acid,and then cleaned up by Waters Oasis HLB and MCX solid phase extraction cartridges.The separation was performed on an AtlantisT3chromatographic column with gradient elution using methanol and water containing 0.1%formic acid.ESI source performed under positive mode was used for the monitoring of the granddaughter ions.The linear ranges for 12beta2-agonists and 4beta-blockers were 0.005-0.160mg/L,and the correlation coefficients were not less than 0.995.The detection limits of 12beta2-agonists and 4beta-blockers were less than 0.2!g/kg.The pooled standard solution was added into blank urine samples at levels of 0.005,0.01 and 0.02mg/L,and the recovery rates were ranged from 41.2%to 88.2%with the relative standard deviations of 12.7%-19.2%.The method is easy,fast,sensitive,and suitable for confirmation and quantification of 12beta2-agonists and 4beta-blockers in urine samples.
中文关键词	液相色谱-质谱法 ; beta2-受体激动剂 ; beta-受体阻断剂 ; 尿液 ; 固相萃取
英文关键词	High performance liquid chromatography-ion trap mass spectrometry beta2-Agonists beta-Blockers Urine Solid phase extraction
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CLINICAL NEUROLOGY
WOS研究方向	Neurosciences & Neurology
CSCD记录号	CSCD:4270680
来源机构	中国农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/226378
作者单位	1.中国农业大学动物医学院, 北京 100193, 中国; 2.中国人民解放军第二炮兵总医院心血管内科, 北京 100088, 中国; 3.中国疾病预防控制中心营养与食品安全所化学污染与健康安全重点实验室, 北京 100021, 中国
推荐引用方式 GB/T 7714	范赛,邹建宏,苗虹,等. 液相色谱-三级质谱法分析尿液中beta2-受体激动剂及beta-受体阻断剂多组分残留[J]. 中国农业大学,2011,39(8):1153-1158.
APA	范赛,邹建宏,苗虹,吴永宁,&赵云峰.(2011).液相色谱-三级质谱法分析尿液中beta2-受体激动剂及beta-受体阻断剂多组分残留.分析化学,39(8),1153-1158.
MLA	范赛,et al."液相色谱-三级质谱法分析尿液中beta2-受体激动剂及beta-受体阻断剂多组分残留".分析化学 39.8(2011):1153-1158.