干旱区生态环境知识资源中心(Arid): 生物基质中全氟辛酸与全氟辛烷磺酸及其前体化合物的超高效液相色谱-串联质谱法研究

Arid

	生物基质中全氟辛酸与全氟辛烷磺酸及其前体化合物的超高效液相色谱-串联质谱法研究
其他题名	Investigation of Perfluorooctanoic Acid and Perfluorooctanesulfonate and Their Precursors in Biological Matrix by Ultra Performance Liquid Chromatography and Tandem Mass Spectrometry
	王雨昕; 李敬光; 赵云峰; 郭斐斐; 吴永宁
来源期刊	分析化学
ISSN	0253-3820
出版年	2011
卷号	39 期号:8 页码:1134-1140
中文摘要	建立了超高效液相色谱-串联三重四级杆质谱(UPLC-MS/MS)测定血清基质与肝匀浆基质中全氟辛酸与全氟辛烷磺酸及其前体化合物的方法。相对应的生物基质中的目标化合物分别用水或甲醇超声提取后,过固相萃取柱(Waters Oasis?WAX 3cc)进行净化,并分别使用甲醇洗脱前体化合物,9%氨水-甲醇洗脱全氟辛酸与全氟辛烷磺酸。经Waters ACQUITYTMBEH C18色谱柱(50mm2.1mm1.7!m)分离,在电喷雾负离子源和多反应监测模式下进行测定,采用内标法进行定量分析。4种目标化合物在两种生物基质中的加标回收率为94.6%~128.4%;相对标准偏差为2.5%~14.9%;检出限为0.01~0.42!g/L;定量限为0.04~1.5!g/L。本方法简单、快速,准确度和灵敏度高,满足生物基质中全氟辛酸与全氟辛烷磺酸的代谢表征和暴露来源研究的需要。
英文摘要	An original method based on ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)was developed for the determination of perfluorooctanoic acid(PFOA),perfluorooctanesulfonate(PFOS)and their precursors in biological matrix.Target compounds were extracted with water or methanol in corresponding biological matrix by ultrasonic.Then the extract was purified by solid phase extraction(Waters Oasis?WAX 6cc),after that the precursors compounds were eluted with methanol and PFOA and PFOS were eluted with 9%NH4OH in methanol.Finally the target compounds were separated on a Waters ACQUITY~(TM)BEH C_(18)column(50mm2.1mm1.7!m)and detected by negative electrospray ionization mass spectrometry in multiple reaction monitoring mode.The average recoveries for four target compounds in two biological matrixs were 94.6%-128.4%by using internal standard method and the relative standard deviations were 2.5%-14.9%.For the four target compounds in two biological matrixs,the limits of detection of the method were in the range of 0.01-0.42!g/L and the limits of quantification were in the range of 0.04-1.5!g/L.With high accuracy and sensitivity,the method was simple and rapid,and can apply to evaluate the metabolic characterization and the source of exposure for PFOA and PFOS in biological matrix.
中文关键词	超高效液相色谱-串联三重四级杆质谱(UPLC-MS/MS) ; 生物基质 ; 固相萃取 ; 全氟辛酸 ; 全氟辛烷磺酸 ; 前体化合物
英文关键词	Ultra performance liquid chromatography and tandem mass spectrometry Biological matrix Solid phase extraction Perfluorooctanoic acid Perfluorooctanesulfonate Precursors compounds
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PUBLIC ENVIRONMENTAL OCCUPATIONAL HEALTH
WOS研究方向	Public, Environmental & Occupational Health
CSCD记录号	CSCD:4270677
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/226377
作者单位	中国疾病预防控制中心营养与食品安全所, 北京 100050, 中国
推荐引用方式 GB/T 7714	王雨昕,李敬光,赵云峰,等. 生物基质中全氟辛酸与全氟辛烷磺酸及其前体化合物的超高效液相色谱-串联质谱法研究[J],2011,39(8):1134-1140.
APA	王雨昕,李敬光,赵云峰,郭斐斐,&吴永宁.(2011).生物基质中全氟辛酸与全氟辛烷磺酸及其前体化合物的超高效液相色谱-串联质谱法研究.分析化学,39(8),1134-1140.
MLA	王雨昕,et al."生物基质中全氟辛酸与全氟辛烷磺酸及其前体化合物的超高效液相色谱-串联质谱法研究".分析化学 39.8(2011):1134-1140.